Methamphetamine causes widespread apoptosis in the mouse brain: evidence from using an improved TUNEL histochemical method
Methamphetamine causes widespread apoptosis in the mouse brain: evidence from using an improved TUNEL histochemical method
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DOI：
10.1016/s0169-328x(01)00184-x
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发表时间：
2001-09-10
期刊：
影响因子：
--
通讯作者：
Cadet, JL
中科院分区：
文献类型：
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作者：
Deng, XL;Wang, Y;Cadet, JL
末端脱氧核苷酸转移酶（TdT）介导的dNTP缺口末端标记（TUNEL）组织化学是一种敏感的方法来暴露凋亡细胞中的DNA链断裂。但是很难在滑动安装的部分上进行。通过使用80 degreesC/0.5%Triton X-100预处理，我们已经开发了一种TUNEL组织化学方法，具有高度的特异性和敏感性，使用缺血大鼠脑切片。之后。在小鼠大脑中研究了甲基苯丙胺（METH）诱导的神经元死亡。结果表明，单次注射40 mg/kg METH可导致数个脑区的神经元死亡，包括纹状体、皮质（额叶、顶叶和梨状）、灰核、内侧缰核。和海马这些结果进一步证实了在非多巴胺能神经元中存在MET诱导的有害作用。这些发现的意义进行了讨论。(C)2001 Elsevier Science B. V.保留所有权利。
Terminal deoxynucleotidyl transferase (TdT)-mediated dNTP nick end labeling (TUNEL) histochemistry is a sensitive method to expose DNA strand breaks in apoptotic cells. but it is difficult to conduct on slide-mounted sections. By using a 80 degreesC/0.5% Triton X-100 pretreatment, we have developed a TUNEL histochemical approach with high specificity and sensitivity using sections from ischemic rat brains. Thereafter. methamphetamine (METH)-induced neuronal death was investigated in mice brains. The results showed that a single injection of 40 mg/kg METH caused neuronal death in several brain areas including the striatum, cortex (frontal, parietal, and piriform), indusium griseum, medial habenular nucleus. and hippocampus. These results further confirmed the presence of METH-induced deleterious effects in nondopaminergic neurons. The significance of these findings is also discussed. (C) 2001 Elsevier Science B.V. All rights reserved.