Validation of optical voltage reporting by the genetically encoded voltage indicator VSFP-Butterfly from cortical layer 2/3 pyramidal neurons in mouse brain slices.
Validation of optical voltage reporting by the genetically encoded voltage indicator VSFP-Butterfly from cortical layer 2/3 pyramidal neurons in mouse brain slices.
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DOI：
10.14814/phy2.12468
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发表时间：
2015-07-29
影响因子：
2.5
通讯作者：
Knöpfel T
中科院分区：
文献类型：
--
作者：
Empson RM;Goulton C;Scholtz D;Gallero-Salas Y;Zeng H;Knöpfel T
了解行为是如何从脑电活动中产生的是神经科学的最终目标之一。为了实现这一目标，我们需要大规模记录特定神经元回路的电活动的方法。一种非常有前途的方法是使用膜电压瞬变的光学报告，特别是如果电压报告基因靶向特定的神经元群体。以这种方式定位允许记录和解释群体信号，而不会对神经元多样性视而不见。在这里，我们评估了电压敏感的荧光蛋白，VSFP蝴蝶2.1，一种遗传编码的电压指示器（GEVI），用于监测小鼠脑切片中2/3层皮质锥体神经元的电活动。标准宽场荧光和双光子成像显示膜电压瞬变的稳健的、高信噪比的读出，其本质上主要是突触的，并且可以被解析为突触连接的层2/3神经元的离散区域。我们发现，有针对性地表达这种GEVI在皮质提供了一个灵活的和有前途的工具，L2/3皮质网络功能的分析。
Understanding how behavior emerges from brain electrical activity is one of the ultimate goals of neuroscience. To achieve this goal we require methods for large-scale recording of the electrical activity of specific neuronal circuits. A very promising approach is to use optical reporting of membrane voltage transients, particularly if the voltage reporter is genetically targeted to specific neuronal populations. Targeting in this way allows population signals to be recorded and interpreted without blindness to neuronal diversity. Here, we evaluated the voltage-sensitive fluorescent protein, VSFP Butterfly 2.1, a genetically encoded voltage indicator (GEVI), for monitoring electrical activity of layer 2/3 cortical pyramidal neurons in mouse brain slices. Standard widefield fluorescence and two-photon imaging revealed robust, high signal-to-noise ratio read-outs of membrane voltage transients that are predominantly synaptic in nature and can be resolved as discrete areas of synaptically connected layer 2/3 neurons. We find that targeted expression of this GEVI in the cortex provides a flexible and promising tool for the analysis of L2/3 cortical network function.