Genetically Encoded Phosphine Ligand for Metalloprotein Design
Genetically Encoded Phosphine Ligand for Metalloprotein Design
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DOI：
10.1021/jacs.2c09683
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发表时间：
2022-11-23
影响因子：
15
通讯作者：
Chen, Yong-Xiang
中科院分区：
文献类型：
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作者：
Duan, Hua-Zhen;Hu, Cheng;Chen, Yong-Xiang
膦配体由于其独特的电子和空间性质而成为交叉偶联反应中最重要的一类配体。然而，金属蛋白通常依赖于氮、硫或氧配体。在这里，我们报告的P3BF，其中包含一个生物相容性的硼烷保护的膦，到蛋白质的基因掺入。该步骤之后是简单的一锅策略，在水性和有氧条件下进行脱硼和钯配位。基因编码的膦配体P3BF应显着扩大我们的能力，设计功能金属蛋白。
Phosphine ligands are the most important class of ligands for cross-coupling reactions due to their unique electronic and steric properties. However, metalloproteins generally rely on nitrogen, sulfur, or oxygen ligands. Here, we report the genetic incorporation of P3BF, which contains a biocompatible borane-protected phosphine, into proteins. This step is followed by a straightforward one-pot strategy to perform deboronation and palladium coordination in aqueous and aerobic conditions. The genetically encoded phosphine ligand P3BF should significantly expand our ability to design functional metalloproteins.