Peptide nanowires for coordination and signal transduction of peroxidase biosensors to carbon nanotube electrode arrays.
Peptide nanowires for coordination and signal transduction of peroxidase biosensors to carbon nanotube electrode arrays.
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DOI：
10.1016/j.bios.2007.06.019
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发表时间：
2007-11
影响因子：
12.6
通讯作者：
Joanne I. Yeh;A. Lazareck;J. Kim;J. Xu;S. Du
中科院分区：
文献类型：
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作者：
Joanne I. Yeh;A. Lazareck;J. Kim;J. Xu;S. Du
一种将肽金属化作为电子信号管道，在氧化还原酶和碳纳米管电极之间架起桥梁的策略已经被开发出来，并取得了增强的效果。同时，开发了一种将生物元件连接到碳纳米管尖端的协议，以优化氧化还原酶和碳纳米管电极阵列之间的接触和几何形状。由 33 个氨基酸组成的肽纳米线由亮氨酸拉链基序组成，经过突变可结合二价金属，从而赋予肽导电性。肽的硫醇盐与 NADH 过氧化物酶的磺酸之间的反应形成肽-酶组装体，该组装体完全准备好将电子从酶活性位点转导到电极。扫描电子显微镜显示组件按照设计专门固定和连接到碳纳米管电极的尖端。等温滴定量热法和质谱法表明每条肽链结合至少三种金属的结合化学计量。总的来说，这些结果强调了当生物传感器的信号转导单元通过定向肽化学排列时可以获得的增益。
A strategy of metallizing peptides to serve as conduits of electronic signals that bridge between a redox enzyme and a carbon-nanotube electrode has been developed with enhanced results. In conjunction, a protocol to link the biological elements to the tips of carbon nanotubes has been developed to optimize contact and geometry between the redox enzyme and the carbon nanotube electrode array. A peptide nanowire of 33 amino acids, comprised of a leucine zipper motif, was mutated to bind divalent metals, conferring conductivity into the peptide. Reaction between a thiolate of the peptide with the sulfenic acid of the NADH peroxidase enzyme formed a peptide–enzyme assembly that are fully primed to transduce electrons out of the enzyme active site to an electrode. Scanning electron microscopy shows immobilization and linking of the assembly specifically to the tips of carbon nanotube electrodes, as designed. Isothermal titration calorimetry and mass spectrometry indicate a binding stoichiometry of at least three metals bound per peptide strand. Overall, these results highlight the gain that can be achieved when the signal tranducing units of a biosensor are aligned through directed peptide chemistry.