Partiti-like viruses from African armyworm increase larval and pupal mortality of a novel host: the Egyptian cotton leafworm.
Partiti-like viruses from African armyworm increase larval and pupal mortality of a novel host: the Egyptian cotton leafworm.
复制标题
来自非洲粘虫的帕蒂蒂样病毒会增加新宿主埃及棉叶虫的幼虫和蛹死亡率。
DOI：
10.1002/ps.6771
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发表时间：
2022
影响因子：
4.1
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Xu P
背景利用一个物种的微生物来管理不同的害虫物种的一般原则在大规模释放携带来自果蝇的沃尔巴克氏杆菌的蚊子方面有着明确的先例。新技术将促进发现可以以类似方式使用的微生物。此前，我们在非洲粘虫中发现了三种新的节段性病毒。为了进一步研究昆虫病毒的寄主转移作为一种潜在的害虫管理工具的实用性和一致性，我们测试了颗粒状病毒与另一种新宿主埃及棉花叶虫(Spotor Tera Littoraris)之间的相互作用。结果发现3种颗粒状病毒似乎都对新宿主有害。导致幼虫和幼虫死亡率增加。未观察到对寄主繁殖力的影响，当另一种病原物杆状病毒SpliNPV攻击时，部分样病毒感染不影响寄主的敏感性。感染和未感染部分样病毒的转录组分析。结果表明，病毒对寄主基因表达谱有一定影响。Littoris，但它们通过影响与免疫(Jak-STAT/Toll和IMD)和生殖(胰岛素信号/昆虫激素)相关的途径而影响另外两种夜蛾的不同途径。这些结果表明，这些颗粒状病毒与新寄主之间存在寄生关系，这表明通过新病毒的人工寄主转移，可能会有新的害虫管理策略和用途。©2021化学工业学会。
BACKGROUNDThe general principle of using microbes from one species to manage a different pest species has a clear precedent in the large‐scale release of mosquitoes carrying aWolbachiabacterium derived fromDrosophilaflies. New technologies will facilitate the discovery of microbes that can be used in a similar way. Previously, we found three novel partiti‐like viruses in the African armyworm (Spodoptera exempta). To investigate further the utility and consistency of host shift of insect viruses as a potential pest management tool, we tested the interaction between the partiti‐like viruses and another novel host, the Egyptian cotton leafworm (Spodoptera littoralis).RESULTWe found that all three partiti‐like viruses appeared to be harmful to the novel hostS. littoralis, by causing increased larval and pupal mortality. No effect was observed on host fecundity, and partiti‐like virus infection did not impact host susceptibility when challenged with another pathogen, the baculovirus SpliNPV. Transcriptome analysis of partiti‐like virus‐infected and noninfectedS. littoralisindicated that the viruses could impact host gene‐expression profiles ofS. littoralis, but they impact different pathways to the two otherSpodopteraspecies through effects on pathways related to immunity (Jak–STAT/Toll and Imd) and reproduction (insulin signaling/insect hormones).CONCLUSIONTaken together with the previous findings in the novel hostS. frugiperda, these results indicate a parasitic relationship between the partiti‐like viruses and novel insect hosts, suggesting a possible use and novel pest management strategy through the artificial host shift of novel viruses. © 2021 Society of Chemical Industry.