Human epidermal plasminogen activator. Characterization, localization, and modulation.
Human epidermal plasminogen activator. Characterization, localization, and modulation.
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人表皮纤溶酶原激活剂。
DOI：
10.1016/0014-4827(85)90093-x
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发表时间：
1985
影响因子：
3.7
通讯作者：
Lazarus,GS
中科院分区：
文献类型：
--
作者：
Morioka,S;Jensen,PJ;Lazarus,GS
利用生物化学和免疫细胞化学方法，我们研究了原代人表皮细胞培养的纤溶酶原激活剂（PA）。抗人尿PA（尿激酶）的兔抗体抑制角化细胞培养物中PA活性的96%。免疫印迹和免疫双扩散分析的角质形成细胞PA与抗尿激酶抗体证实，表皮PA的尿激酶型。人角质形成细胞培养物与抗尿激酶抗体的免疫细胞化学研究显示PA的两个特征性染色模式。首先，在亚融合集落的前进边缘处的细胞以颗粒图案进行胞质染色。在用刀片切割受伤的融合表皮细胞培养物的迁移边缘观察到类似的染色。PA染色的诱导是独立的细胞分裂。其次，位于集落表面的分化的表皮细胞在质膜或整个细胞中均匀染色。高度分化的、自发脱落的细胞通常被抗尿激酶抗体染色得很重。这些免疫细胞化学实验表明，PA的表达是高度调节的人表皮细胞。具体而言，PA表达似乎与细胞分化和细胞运动在扩大或受伤的角质形成细胞集落。
Using biochemical and immunocytochemical approaches, we have investigated the plasminogen activator (PA) of primary human epidermal cell cultures. A rabbit antibody raised against human urinary PA (urokinase) inhibited ⩾96% of the PA activity in the keratinocyte cultures. Immunoblot and double immunodiffusion analyses of keratinocyte PA with anti-urokinase antibody confirmed that epidermal PA was of the urokinase type. Immunocytochemical investigation of human keratinocyte cultures with anti-urokinase antibody revealed two characteristic staining patterns for PA. First, cells at the advancing edge of subconfluent colonies were cytoplasmically stained in a granular pattern. Similar staining was observed at the migrating edges of confluent epidermal cell cultures that had been wounded by cutting with a blade. This induction of PA staining was independent of cell division. Secondly, differentiated epidermal cells located on the surface of colonies were stained either at the plasma membrane or homogeneously throughout the cell. The highly differentiated, spontaneously shed cells were usually very heavily stained by anti-urokinase antibody. These immunocytochemical experiments suggest that PA expression is highly regulated in human epidermal cells. Specifically, PA expression appears to be related to cellular differentiation and to cell movement in expanding or wounded keratinocyte colonies.