Contribution of nuclear actin to transcription regulation.
Contribution of nuclear actin to transcription regulation.
复制标题
DOI：
10.1016/j.gdata.2015.04.009
复制
发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Harata M
中科院分区：
文献类型：
--
作者：
Yamazaki S;Yamamoto K;Harata M
肌动蛋白是细胞骨架的重要组成部分，在细胞迁移、黏附、极性和形状改变等多种细胞功能中发挥重要作用。过去几十年的研究表明，肌动蛋白也存在于细胞核中。然而，到目前为止，核肌动蛋白的功能和性质仍然不清楚。最近，我们发现标记了EYFP并与核定位信号(EYFP-NLS-Actin)融合的肌动蛋白在细胞中形成了可见的丝状(F)-肌动蛋白束。为了进一步了解受核肌动蛋白影响的单个基因，我们使用微阵列分析来确定EYFP-NLS-肌动蛋白表达导致HeLa细胞中RNA表达水平的变化。我们的结果表明，核肌动蛋白在基因激活中发挥作用，而不是在基因抑制中发挥作用。这些数据已保存在基因表达总览(GEO)数据库中，登录号为GSE59799。
Actin, an integral component of the cytoskeleton, plays crucial roles in a variety of cell functions, including cell migration, adhesion, polarity and shape change. Studies performed during the last couple of decades have revealed that the actin also exists in the nucleus. However, the function and properties of nuclear actin remained elusive so far. Recently, we showed that an actin tagged with EYFP and fused with a nuclear localization signal (EYFP-NLS-actin) formed visible filamentous (F)-actin bundles in cells. To obtain further details about the individual genes that are affected by the nuclear actin, we have used the microarray analysis to determine the changes in the expression levels of RNAs in HeLa cells as a result of EYFP-NLS-actin expression. Our results suggest that the nuclear actin plays a role in the activation of genes rather than their repression. The data has been deposited in the Gene Expression Omnibus (GEO) database under the accession number GSE59799.