Identification of QTLs for cold tolerance at seedling stage in rice (Oryza sativa L.) using two distinct methods of cold treatment
Identification of QTLs for cold tolerance at seedling stage in rice (Oryza sativa L.) using two distinct methods of cold treatment
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DOI：
10.1007/s10681-013-0977-0
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发表时间：
2013
期刊：
影响因子：
1.9
通讯作者：
Shaohong Zhang;Jingsheng Zheng;B. Liu;S. Peng;H. Leung;Junliang Zhao;Xiaofei Wang;Tifeng Yang
中科院分区：
文献类型：
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作者：
Shaohong Zhang;Jingsheng Zheng;B. Liu;S. Peng;H. Leung;Junliang Zhao;Xiaofei Wang;Tifeng Yang
冷水灌溉和低温植园生长是评价水稻苗期耐冷性的两种常用方法，对冷敏感的幼苗表现出不同的伤害症状。然而，迄今为止还没有人系统地剖析两种低温环境下水稻苗期耐冷性的差异。本研究以耐冷粳稻品种“丽江新团黑谷”和冷敏感粳稻品种“三黄战2号”杂交而成的重组自交系（RIL）群体为研究对象。冷敏苗在低温植园生长过程中表现出冷水灌溉后叶片泛黄和叶片滚叶的现象。叶片黄变与卷叶呈极显著相关，但相关系数较低。以叶片黄化和幼苗成活率作为冷水灌溉后耐寒指标，在1、6、9和12号染色体上共检测到4个数量性状位点（qtl）；以叶片卷曲和幼苗成活率作为低温植草生长过程中耐寒指标，在7、8、9、11和12号染色体上共检测到5个数量性状位点（qtl）。在两种低温环境下，采用不同的评价指标对qcts -9和qcts -12两个qtl进行检测。我们的研究结果表明，水稻在苗期的耐寒机制在两种环境中存在差异，但共同QTL的检测表明，在耐寒代谢途径中存在重叠。这两个常见的qtl在水稻育种中具有潜在的应用价值。
Cold water irrigation and growth in low temperature phytotron are two commonly used methods to evaluate cold tolerance of rice at the seedling stage and the cold sensitive seedlings exhibit different injury symptoms, respectively. However, so far no one has systematically dissected the differences of cold tolerance at seedling stage in rice under the two cold environments. We used a recombinant inbred line (RIL) population derived from a cross of a cold-tolerantjaponicacultivar, Lijiangxintuanheigu and a cold sensitiveindicacultivar, Sanhuangzhan-2 for this study. The cold sensitive seedlings exhibited leaf yellowing after cold water irrigation and leaf rolling during growth in the low temperature phytotron. Leaf yellowing and leaf rolling in RILs was significantly correlated, but the correlation coefficient was low. A total of four quantitative trait locus (QTLs) on chromosomes 1, 6, 9 and 12 were detected using leaf yellowing and percent seedling survival as indicators of cold tolerance after cold water irrigation, while five QTLs on chromosomes 7, 8, 9, 11 and 12 were detected using leaf rolling and percent seedling survival as indicators of cold tolerance during growth in the low temperature phytotron. The two QTLs,qCTS-9andqCTS-12were detected using different evaluation indicators under the two cold environments. Our results suggest that rice cold tolerance mechanisms at the seedling stage differ between the two environments, but the detection of common QTL implies the existence of overlap in the metabolic pathways for cold tolerance. The two common QTLs have potential value in rice breeding.