EPIDERMAL GROWTH FACTOR-INDUCED SELECTIVE PHOSPHORYLATION OF CULTURED RAT HEPATOCYTE 55-KD CYTOKERATIN BEFORE FILAMENT REORGANIZATION AND DNA-SYNTHESIS
EPIDERMAL GROWTH FACTOR-INDUCED SELECTIVE PHOSPHORYLATION OF CULTURED RAT HEPATOCYTE 55-KD CYTOKERATIN BEFORE FILAMENT REORGANIZATION AND DNA-SYNTHESIS
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DOI：
10.1083/jcb.109.4.1665
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发表时间：
1989-10-01
影响因子：
7.8
通讯作者：
MARCEAU, N
中科院分区：
文献类型：
--
作者：
BARIBAULT, H;BLOUIN, R;MARCEAU, N
我们以前曾报道，在胰岛素存在下，地塞米松的培养静止乳鼠肝细胞的培养条件，这不会导致生长激活，诱导选择性增加的49-kD/55-kD细胞角蛋白（CK 49/CK 55）对的合成超过24小时的时间。这种增加的合成与致密细丝网络的形成相一致，这使人想起在细胞周边原位观察到的那些（Marceau，N.，H.巴里博和我。勒鲁-尼科莱特1985.可以的. 63：448-457）。我们在这里首次表明，当胰岛素和地塞米松后加入EGF 48小时，有一个早期的优先磷酸化的CK 55的CK 49/CK 55对，诱导丝重排从细胞周围的细胞质，并随后进入S期和有丝分裂后的滞后期8小时。用CK 49和CK 55单克隆抗体的间接免疫荧光显微镜表明，在EGF处理之前，细胞角蛋白丝主要分布在细胞周边附近，EGF的加入导致它们重组到细胞内主要的细胞质定位。
We have reported previously that the addition of dexamethasone to cultured quiescent suckling rat hepatocytes in the presence of insulin, a culture condition which does not cause growth activation, induces a selective increase in the synthesis of the 49-kD/55-kD cytokeratin (CK49/CK55) pair over a 24-h period. This increased synthesis coincides with the formation of dense filament networks reminiscent of those observed in situ at the cell periphery (Marceau, N., H. Baribault, and I. Leroux-Nicollet. 1985. Can. J. Biochem. Cell Biol. 63:448-457). We show here for the first time that when EGF is added 48 h after insulin and dexamethasone, there is an early preferential phosphorylation of the CK55 of the CK49/CK55 pair, an induced filament rearrangement from the cell periphery to the cytoplasm, and a subsequent entry into S phase and mitosis after a lag period of 8 h. Indirect immunofluorescence microscopy with monoclonal antibodies to CK49 and CK55 indicate that, while before EGF treatment the cytokeratin filaments were mainly distributed near the cell periphery, the addition of EGF resulted in their reorganization to a predominantly cytoplasmic localization within