Subseafloor microbial communities in methane hydrate-bearing sediment at two distinct locations (ODP Leg204) in the cascadia margin.
Subseafloor microbial communities in methane hydrate-bearing sediment at two distinct locations (ODP Leg204) in the cascadia margin.
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DOI：
10.1264/jsme2.me08514
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发表时间：
2008-12
影响因子：
2.2
通讯作者：
T. Nunoura;F. Inagaki;M. Delwiche;F. Colwell;K. Takai
中科院分区：
文献类型：
--
作者：
T. Nunoura;F. Inagaki;M. Delwiche;F. Colwell;K. Takai
采用16 SrRNA基因克隆测序和荧光定量PCR技术，对大洋钻探计划（ODP）第204航次（2005 - 2007）在卡斯卡迪亚陆缘南海角湾（SHR）采集的深海底沉积物中的原核生物群落进行了分析。微生物群落来自SHR上具有对比地质特征的站点：站点1244和1245（位于山脊的侧翼，富含水合物的沉积物）和站点1251（位于SHR的斜坡盆地，缺乏水合物的沉积物）。沉积物中16 S rRNA基因的总拷贝数和古生菌16 S rRNA基因在16 S rRNA基因群落中所占的比例在斜坡盆地大于SHR侧翼。使用两种不同的PCR引物集，在站点1251（4.5 mbsf）的硫酸盐-甲烷过渡带周围的微生物群落结构进行了深入研究。一个相对丰富的分布的16 S rRNA基因序列相关的嗜温产甲烷菌属甲烷culleus被确定在43.2 mbsf的深度，并建议产甲烷菌发生在相对较浅的沉积物区。这项研究表明，16 S rRNA基因克隆分析显示的海底下微生物群落与海底下甲烷水合物矿床没有直接关系。
The prokaryotic communities in deep subseafloor sediment collected during Ocean Drilling Program (ODP) Leg 204 from the South Hydrate Ridge (SHR) on the Cascadia Margin were analyzed by 16S rRNA gene clone sequencing and a fluorescent quantitative PCR technique. The microbial communities came from sites with contrasting geological characteristics on the SHR: sites 1244 and 1245 (located on the flank of the ridge, hydrate-rich sediment) and site 1251 (located on the slope basin of SHR, hydrate-poor sediment). The overall copy numbers of the 16S rRNA gene, and the proportion of archaeal 16S rRNA gene in all 16S rRNA gene community in sediment were larger on the slope basin than on the flank of the SHR. Archaeal community structure around the sulfate-methane transition zone at site 1251 (4.5 mbsf) was intensively investigated using two different PCR primer sets. A relatively abundant distribution of the 16S rRNA gene sequences related to mesophilic methanogen of the genus Methanoculleus was identified at a depth of 43.2 mbsf, and suggested that the methanogens occur in relatively shallow zones of sediment. This study demonstrated that the subseafloor microbial communities shown by 16S rRNA gene clone analyses were not directly associated with subseafloor methane hydrate deposits.