Expression of osteoprotegerin correlates with aggressiveness and poor prognosis of gastric carcinoma
Expression of osteoprotegerin correlates with aggressiveness and poor prognosis of gastric carcinoma
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DOI：
10.1007/s00428-003-0845-8
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发表时间：
2003-08-01
期刊：
影响因子：
3.5
通讯作者：
Yasui, W
中科院分区：
文献类型：
--
作者：
Ito, R;Nakayama, H;Yasui, W
骨保护素（OPG）是肿瘤坏死因子（TNF）受体超家族的一个成员，与破骨细胞生成抑制因子（OOP）相同，是一种可溶性诱饵受体。有研究表明，OPG的表达与乳腺癌和前列腺癌的骨转移有关。在本研究中，我们研究了OPG在胃癌中的表达，采用免疫组织化学和逆转录聚合酶链反应方法，并与临床病理参数进行比较。胃癌细胞株MKN-7和胃癌组织中均检测到OPG mRNA的表达。免疫组化结果显示，OPG在65%（67/103）的胃癌组织中呈强阳性表达，而在非肿瘤性粘膜上皮中未见表达。OPG蛋白在胃癌组织中的表达与肿瘤浸润深度、淋巴结转移及肿瘤分期密切相关。OPG强表达者的预后明显差于OPG弱表达或无表达者。提示OPG可能参与胃癌的发生、侵袭和转移，可能成为胃癌侵袭性的一个新的分子标志物。
Osteoprotegerin (OPG), identical with osteoclastogenesis inhibitory factor, is a member of a subgroup of the tumor necrosis factor (TNF)-receptor superfamily, which functions as a soluble decoy receptor. It has been reported that OPG expression is associated with bone metastasis of cancer of the breast and prostate. In the present study, we examined the expression of OPG in gastric carcinomas using immunohistochemistry and reverse-transcription polymerase chain reaction methods, and compared with clinicopathological parameters. The expression of OPG mRNA was confirmed in a gastric carcinoma cell line (MKN-7) and gastric carcinoma tissues. Immunohistochemically, strongly positive staining of OPG was found in 65% (67/103) of gastric carcinomas, whereas OPG protein was not detected in non-neoplastic mucosal epithelia. The expression of OPG protein in gastric carcinoma tissues correlates significantly with depth of tumor invasion, nodal metastases and advanced tumor stage. Furthermore, the prognosis of the cases with strong OPG expression was significantly worse than those with weak or no expression of OPG. These results suggest that OPG may participate in stomach carcinogenesis, invasion and metastasis, and may serve as a novel molecular marker for aggressive gastric cancer.