Repetitive anodal transcranial direct current stimulation improves neurological recovery by preserving the neuroplasticity in an asphyxial rat model of cardiac arrest
Repetitive anodal transcranial direct current stimulation improves neurological recovery by preserving the neuroplasticity in an asphyxial rat model of cardiac arrest
复制标题
重复阳极经颅直流电刺激通过保留心脏骤停窒息大鼠模型的神经可塑性来改善神经功能恢复
DOI：
10.1016/j.brs.2021.02.008
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发表时间：
2021-02-25
影响因子：
7.7
通讯作者：
Li，Yongqin
中科院分区：
文献类型：
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作者：
Dai，Chenxi;Wang，Jianjie;Li，Yongqin
背景非电击性心律在院外心脏骤停（CA）患者中所占比例越来越大，但预后不良，靶向温度管理（TTM）治疗效果有限。先前的研究表明，重复阳极经颅直流电刺激（tDCS）改善了室颤动物的神经功能结局。在这里，我们研究的有效性tDCS对神经恢复和窒息CA的大鼠模型中的潜在机制。方法心脏复苏后5分钟的未经处理的窒息CA。成功复苏后立即将动物随机分配至3个实验组（n = 12/组，6只雄性）：无处理对照（NTC）组、TTM组和tDCS组。评估复苏后血流动力学、定量脑电图（EEG）、神经功能缺损评分和96小时存活率。收获经历相同实验程序的其他动物的脑组织用于基于酶联免疫测定的神经可塑性相关生物标志物的定量测定，并与假手术大鼠进行比较结果复苏后，tDCS组动物的平均动脉压和左心室射血分数明显高于NTC组，EEG特征也有明显改善，包括加权平均动脉压和左心室射血分数。与NTC和TTM组相比，置换熵和伽马带功率以及神经功能缺损评分和96小时存活率。此外，神经可塑性的生物标志物，包括微管相关蛋白2，生长相关蛋白43，突触后密度蛋白95和突触素，在tDCS组显着较高时相比，NTC和TTM groups.ConclusionIn此大鼠模型窒息CA，复苏后开始重复阳极tDCS改善神经恢复，它可能发挥神经保护作用，通过保存神经可塑性。
BackgroundNon-shockable rhythms present an increasing proportion of out-of-hospital cardiac arrest (CA) patients, but are associated with poor prognosis and received limited therapeutic effect of targeted temperature management (TTM). Previous study showed repetitive anodal transcranial direct current stimulation (tDCS) improved neurological outcomes in animals with ventricular fibrillation. Here, we examine the effectiveness of tDCS on neurological recovery and the potential mechanisms in a rat model of asphyxial CA.MethodCardiopulmonary resuscitation was initiated after 5 min of untreated asphyxial CA. Animals were randomized to three experimental groups immediately after successful resuscitation (n = 12/group, 6 males): no-treatment control (NTC) group, TTM group, and tDCS group. Post resuscitation hemodynamics, quantitative electroencephalogram (EEG), neurological deficit score, and 96-h survival were evaluated. Brain tissues of additional animals undergoing same experimental procedure was harvested for enzyme-linked immunoassay-based quantification assays of neuroplasticity-related biomarkers and compared with the sham-operated rats (n = 6/group).ResultsWe observed that after resuscitation tDCS-treated animals exhibited significantly higher mean arterial pressure and left ventricular ejection fraction than NTC group and showed greatly improved EEG characteristics including weighted-permutation entropy and gamma band power, and neurologic deficit scores and 96-h survival rates compared to NTC and TTM groups. Furthermore, neuroplastic biomarkers including microtubule-associated protein 2, growth-associated protein 43, postsynaptic density protein 95 and synaptophysin, were significantly higher in tDCS group when compared with NTC and TTM groups.ConclusionIn this rat model of asphyxial CA, repetitive anodal tDCS commenced after resuscitation improved neurological recovery, and it may exert a neuroprotective effect by preserving the neuroplasticity.