INCREASE IN ACTIVATED T-CELLS AND REDUCTION IN SUPPRESSOR INDUCER T-CELLS IN SYSTEMIC-SCLEROSIS
INCREASE IN ACTIVATED T-CELLS AND REDUCTION IN SUPPRESSOR INDUCER T-CELLS IN SYSTEMIC-SCLEROSIS
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DOI：
10.1136/ard.49.1.40
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发表时间：
1990-01-01
影响因子：
27.4
通讯作者：
HALLGREN, R
中科院分区：
文献类型：
--
作者：
GUSTAFSSON, R;TOTTERMAN, TH;HALLGREN, R
血液淋巴细胞从37例系统性硬化症的特点是使用单克隆抗体在双色流式细胞仪（荧光激活细胞分选仪（FACS））分析。与30名健康对照相比，患者的辅助性CD 4 +/抑制性/细胞毒性CD 8 + T细胞的比例升高，原因是CD 8+细胞减少。CD 4+和CD 8+细胞表达活化标记的HLA-DR，CD 11b + CD 8+淋巴细胞（抑制性T细胞）的相对数量正常，但绝对计数略有减少。抑制诱导T细胞（定义为CD 45 R + CD 4+细胞）的比例和绝对数量平均仅为对照组中观察到的水平的一半。这些发现与急性期血浆蛋白或血清免疫球蛋白测定的炎症活性无关。活化的T细胞出现在硬化过程的各个阶段，特别是在疾病的早期阶段和职业暴露于硅尘的患者中。高比例的活化T细胞也与小肠功能受损有关，但与皮肤或肺部受累程度无关。抑制性诱导T细胞的丢失在疾病后期和CREST（钙质沉着症、雷诺现象、食管动力障碍、指硬化症、毛细血管扩张症）综合征患者中更为明显。这些数据提供了进一步的证据，参与T细胞介导的免疫系统性硬化症的永久化。
Blood lymphocytes from 37 patients with systemic sclerosis were characterised using monoclonal antibodies in a two colour flow cytometric (fluorescence activated cell sorter (FACS)) analysis. The ratio of helper CD4+ to suppressor/cytotoxic CD8+ T cells was raised in patients compared with the in 30 healthy controls owing to decreased CD8+ cells. In the patients CD4+ and CD8+ cells displayed an increased expression of the activation marked HLA-DR. The relative number of CD11b+ CD8+ lymphocytes (suppressor T cells) was normal, but the calculated absolute counts of this cell type were slightly reduced. The proportions and absolute numbers of suppressor inducer T cells, defined as CD45R+ CD4+ cells, were on average only half the levels observed in controls. These findings were not related to the inflammatory activity as measured by acute phase plasma proteins or serum immunoglobulins. Activated T cells were seen at all stages of the sclerotic process and especially during the early stages of the disease and in patients who had suffered occupational exposure to silica dust. A high proportion of activated T cells was also linked with impaired small intestine function but not with the degree of skin or lung involvement. A loss of suppressor inducer T cells was more pronounced later in the disease and in patients with the CREST (calcinosis, Raynaud''s phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia) syndrome. These data provide further evidence for an involvement of T cell mediated immunity in the perpetuation of systemic sclerosis.