ISOLATION OF THERMOTOLERANT, FERMENTATIVE YEASTS GROWING AT 52-DEGREES-C AND PRODUCING ETHANOL AT 45-DEGREES-C AND 50-DEGREES-C
ISOLATION OF THERMOTOLERANT, FERMENTATIVE YEASTS GROWING AT 52-DEGREES-C AND PRODUCING ETHANOL AT 45-DEGREES-C AND 50-DEGREES-C
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DOI：
10.1007/bf01201874
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发表时间：
1992-05-01
影响因子：
4.1
通讯作者：
MARCHANT, R
中科院分区：
文献类型：
--
作者：
BANAT, IM;NIGAM, P;MARCHANT, R
从印度获得的样品中分离出五种耐热的产酒精酵母培养物。其中2株经鉴定为马克斯克鲁维酵母。这五种细胞都在高达52摄氏度的平板培养物上生长，其中在40摄氏度的液体培养物中生长率最高。当在14.0%（w/v）葡萄糖上生长时，所有菌株都产生相对较高的酒精浓度：45 ℃时为5.7至7.0%（w/v），50 ℃时为5.0至5.5%（w/v）。所有五个菌株发酵稀释糖蜜含有16.0%（w/v）的总糖，产生5.6至6.0%（w/v）的酒精浓度。在糖蜜中添加磷、钾、镁和锰，在40 ℃时，酒精产量增加了13%至20%。 在37 ℃和40 ℃下，补充糖蜜产生的最大酒精量分别为7.5至8.0和6.5至7.0%（w/v）。
Five thermotolerant, alcohol-producing yeast cultures were isolated from samples obtained from India. Two were identified as of Kluyveromyces marxianus. All five grew on plate-cultures up to 52-degrees-C, with maximum growth rates in liquid culture at 40-degrees-C. All produced relatively high alcohol concentrations: 5.7 to 7.0% (w/v) at 45-degrees-C and 5.0 to 5.5% (w/v) at 50-degrees-C when growing on 14.0% (w/v) glucose. All five isolates fermented diluted molasses containing 16.0% (w/v) total sugars, producing 5.6 to 6.0% (w/v) alcohol concentrations. Supplementing the molasses with P, K, Mg and Mn resulted in a 13 to 20% increase in alcohol production at 40-degrees-C. The maximum amounts of alcohol produced on supplemented molasses were 7.5 to 8.0 and 6.5 to 7.0% (w/v) at 37-degrees-C and 40-degrees-C, respectively.