Aspergillus fumigatus conidia induce a Th1-type cytokine response
Aspergillus fumigatus conidia induce a Th1-type cytokine response
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DOI：
10.1086/514157
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发表时间：
1997-12-01
影响因子：
6.4
通讯作者：
Savary, CA
中科院分区：
文献类型：
--
作者：
Grazziutti, ML;Rex, JH;Savary, CA
本文研究了人外周血单个核细胞（MNC）对烟曲霉菌的体外增殖反应。15个供体对烟曲霉分生孢子显示出显著的反应，刺激指数（SI）在4和193之间。相反，所有供体对白色念珠菌芽生孢子显示出阳性反应（SI在10和224之间）。尽管对A.在烟曲霉分生孢子中，当在1-2周的时间内重新测试时，特定个体的反应是稳定的。与未受刺激的细胞相比，经MNC刺激的烟曲霉分生孢子含有增加的干扰素-γ、粒细胞-巨噬细胞集落刺激因子、肿瘤坏死因子-α和白细胞介素（IL）-2，但不含IL-10或IL-4。烟曲霉诱导淋巴细胞表面粘附/激活相关分子的表达。这些结果表明，淋巴细胞可能有助于宿主防御曲霉菌产生的Th 1型反应。
The response of human peripheral blood mononuclear cells (MNC) to Aspergillus fumigatus in vitro was evaluated, In studies of the proliferative response of MNC from 18 healthy donors to heat-killed A. fumigatus conidia, 15 displayed a significant response, with a stimulation index (SI) between 4 and 193, In contrast, all donors displayed a positive response to Candida albicans blastoconidia (SI ranged from 10 to 224). Despite the variability in reactivity to A. fumigatus conidia, the response of a particular individual was stable when retested over periods of 1-2 weeks, Supernatant from cocultures of A. fumigatus conidia with MNC contained increased levels of interferon-gamma, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-alpha, and interleukin (IL)-2, compared with unstimulated cells, but not IL-10 or IL-4, In addition, A. fumigatus induced lymphocyte surface expression of adhesion/activation-associated molecules. These results suggest that lymphocytes may contribute to host defense against Aspergillus by generating a Th1-type response.