Conversion of mammalian Muller glial cells into a neuronal lineage by in vitro aggregate-culture
Conversion of mammalian Muller glial cells into a neuronal lineage by in vitro aggregate-culture
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DOI：
10.1016/j.bbrc.2006.10.072
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发表时间：
2006-12-15
影响因子：
3.1
通讯作者：
Tano, Yasuo
中科院分区：
文献类型：
--
作者：
Kubota, Akira;Nishida, Kohji;Tano, Yasuo
Muller神经胶质细胞是哺乳动物视网膜上主要的神经胶质细胞。在这里，我们报道这些胶质细胞可以通过体外聚集培养重定向到神经元谱系。大鼠和猕猴穆勒神经胶质细胞不表达神经元标记物，除非转移到粘附条件下。此外，这种表达只发生在血小板衍生生长因子和丙戊酸存在的情况下。我们比较了正常单层培养和聚集培养，发现大鼠Muller胶质细胞只能在无黏附条件下向神经元分化。然而，Muller胶质细胞在体外不表达光受体标记物。大鼠Muller胶质细胞移植到视网膜下间隙（视网膜特异性生态位）后，表达光受体特异性标记物视蛋白（RET-P1）。我们证明了哺乳动物Muller胶质细胞作为光感受器来源的潜力，这可能有助于治疗退行性视网膜疾病，如视网膜色素变性。(c) 2006爱思唯尔公司版权所有。
Mammalian Muller glial cells are major glial cells in the retina. Here we report that these glial cells can be redirected towards a neuronal lineage by an aggregate-culture in vitro. Rat and macaque Muller glial cells did not express neuronal markers except after transfer to adhesive conditions. Furthermore, this expression could only take place in the presence of platelet-derived growth factor and valproic acid. We compared a normal monolayer-culture and an aggregate-culture, and rat Muller glial cells could only differentiate into neurons under non-adhesive conditions. However, Muller glial cells did not express the photoreceptor markers in vitro. After transplantation into the subretinal space, a retina-specific niche, rat Muller glial cells expressed the photoreceptor-specific marker, opsin (RET-P1). We demonstrate the potential of mammalian Muller glial cells as a source of photoreceptors, which may possibly contribute to the treatment of degenerative retinal diseases such as retinitis pigmentosa. (c) 2006 Elsevier Inc. All rights reserved.