Silencing the circadian clock gene Clock using RNAi reveals dissociation of the circatidal clock from the circadian clock in the mangrove cricket.
Silencing the circadian clock gene Clock using RNAi reveals dissociation of the circatidal clock from the circadian clock in the mangrove cricket.
复制标题
使用 RNAi 沉默生物钟基因 Clock，揭示了红树林蟋蟀的生物钟与生物钟的分离。
DOI：
10.1016/j.jinsphys.2014.06.012
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发表时间：
2014
影响因子：
2.2
通讯作者：
S.G.
中科院分区：
文献类型：
--
作者：
Takekata;H.;Numata;H.;Shiga;S.;Goto;S.G.
产生昼夜节律的时钟是否与昼夜节律钟具有相同的元素还没有完全了解。红树林蟋蟀Apteronemobius asahinai在其运动活动中同时表现出两种内源性节律；昼夜节律产生活跃和不活跃的阶段，昼夜节律通过抑制主观白天的活动来改变活动水平。在本研究中，我们沉默了生物钟的一个主控基因——生物钟（inA）。asahinai利用RNAi研究了生物钟和生物钟之间的联系。在注射了clk双链RNA （dsClk）的蟋蟀中，clkmrna的丰度降低到对照蟋蟀的一半。dsclkin2还降低了另一个生物钟基因周期（per）的mRNA丰度，并减弱了昼夜振荡inpermRNA的表达。在恒定条件下对运动节律的检查显示，沉默clk表达后，昼夜节律改变被破坏，但周期节律未受影响。对照组和注射了dsClk的蟋蟀的自由运行周期没有明显变化。我们的研究结果表明，生物钟对生物钟至关重要，但对生物钟却不是必需的。根据这些结果，我们提出a的周期节律。asahinai是由时钟驱动的，其分子成分与昼夜节律钟不同。
Whether a clock that generates a circatidal rhythm shares the same elements as the circadian clock is not fully understood. The mangrove cricket,Apteronemobius asahinai, shows simultaneously two endogenous rhythms in its locomotor activity; the circatidal rhythm generates active and inactive phases, and the circadian rhythm modifies activity levels by suppressing the activity during subjective day. In the present study, we silencedClock(Clk), a master gene of the circadian clock, inA. asahinaiusing RNAi to investigate the link between the circatidal and circadian clocks. The abundance ofClkmRNA in the crickets injected with double-stranded RNA ofClk(dsClk) was reduced to a half of that in control crickets. dsClkinjection also reduced mRNA abundance of another circadian clock geneperiod(per) and weakened diel oscillation inpermRNA expression. Examination of the locomotor rhythms under constant conditions revealed that the circadian modification was disrupted after silencingClkexpression, but the circatidal rhythm remained unaffected. There were no significant changes in the free-running period of the circatidal rhythm between the controls and the crickets injected with dsClk. Our results reveal thatClkis essential for the circadian clock, but is not required for the circatidal clock. From these results we propose that the circatidal rhythm ofA. asahinaiis driven by a clock, the molecular components of which are distinct from that of the circadian clock.