OPTIMIZING COUPLED ENZYME ASSAYS

OPTIMIZING COUPLED ENZYME ASSAYS
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DOI:
10.1016/0003-2697(79)90055-1
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发表时间:
1979-01-01
影响因子:
2.9
通讯作者:
CLELAND, WW
CLELAND, WW
中科院分区:
生物学4区
文献类型:
--
作者:
CLELAND, WW

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Equations are given for minimizing the cost and calculating the maximum practical rates for coupled assays when 2 or more coupling enzymes are involved, and a lag of a predetermined size can be tolerated. The lag caused by obligatory mutarotation of an intermediate is also calculated.