Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2-13C]dihydroxyacetone
Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2-13C]dihydroxyacetone
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DOI：
10.1002/mrm.26525
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发表时间：
2017-01-01
影响因子：
3.3
通讯作者：
Merritt, Matthew
中科院分区：
文献类型：
--
作者：
Marco-Rius, Irene;von Morze, Cornelius;Merritt, Matthew
PurposeTo调查急性变化后，无论是果糖或葡萄糖在体内肝脏和肾脏中的葡萄糖代谢，使用超极化[2-C-13] dihydroxyaceton.MethodsSpatially注册，动态，multislice磁共振波谱获得的代谢产物的[2-C-13]dihydroxyacetone在肝脏和肾脏。在三个时间点：0、70和140分钟，在13只禁食大鼠中探测代谢。60 min时，分别静脉注射果糖（n=5）或葡萄糖（n=4）0.8g/kg，诱发急性反应。对照组（n=4）没有收到碳水化合物challenge.Results10分钟果糖输注后，水平的[2-C-13]磷酸烯醇丙酮酸和[2-C-13]甘油-3-磷酸在肝脏减半：51%（P=0.0010）和47%（P=0.0001）的基线，分别。70分钟后，水平恢复到基线水平。葡萄糖激发没有显著改变信号，重复给予二羟丙酮成像团也没有显著改变信号。在肾脏中，没有统计学上显着的变化后，糖输液后检测到的甘油-3-磷酸信号增加20%，果糖注射后10和80分钟之间（P=0.0028）。观察到的影响可能包括ATP耗竭和糖酵解中间体的未标记池大小的变化。Magn Reson Med 77：65-73，2017. (c)2016年国际医学磁共振学会
PurposeTo investigate acute changes in glucose metabolism in liver and kidneys in vivo after a bolus injection of either fructose or glucose, using hyperpolarized [2-C-13]dihydroxyacetone.MethodsSpatially registered, dynamic, multislice MR spectroscopy was acquired for the metabolic products of [2-C-13]dihydroxyacetone in liver and kidneys. Metabolism was probed in 13 fasted rats at three time points: 0, 70, and 140min. At 60min, rats were injected intravenously with fructose (n=5) or glucose (n=4) at 0.8g/kg to initiate acute response. Controls (n=4) did not receive a carbohydrate challenge.ResultsTen minutes after fructose infusion, levels of [2-C-13]phosphoenolpyruvate and [2-C-13]glycerol-3-phosphate halved in liver: 51% (P=0.0010) and 47% (P=0.0001) of baseline, respectively. Seventy minutes later, levels returned to baseline. The glucose challenge did not alter the signals significantly, nor did repeated administration of the dihydroxyacetone imaging bolus. In kidneys, no statistically significant changes were detected after sugar infusion other than a 20% increase of the glycerol-3-phosphate signal between 10 and 80min after fructose injection (P=0.0028).ConclusionHyperpolarized [2-C-13]dihydroxyacetone detects a real-time, transient metabolic response of the liver to an acute fructose challenge. Observed effects possibly include ATP depletion and changes in the unlabeled pool sizes of glycolytic intermediates. Magn Reson Med 77:65-73, 2017. (c) 2016 International Society for Magnetic Resonance in Medicine