Allene oxide synthase:: a major control point in Arabidopsis thaliana octadecanoid signalling
Allene oxide synthase:: a major control point in Arabidopsis thaliana octadecanoid signalling
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DOI：
10.1046/j.1365-313x.1998.00245.x
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发表时间：
1998-09-01
期刊：
影响因子：
7.2
通讯作者：
Weiler, EW
中科院分区：
文献类型：
--
作者：
Laudert, D;Weiler, EW
通过对三尖杉烯氧化物合成酶(AOS)基因水平、AOS多肽水平和比酶活性的分析，以及对不同处理后十八烷类顺-12-氧代植物二烯酸(cis-OPDA)和茉莉酸(JA)水平的定量测定，表明AOS是拟南芥十八烷类生物合成的调节位点。伤叶中AOS活性、mRNA和多肽水平局部和系统地升高。OPDA或JA的甲酯(OPDAME、JAME)和冠醚是AOS mRNA、多肽和酶活性的强诱导剂。乙烯利也能诱导AOS活性。水杨酸(SA)是AOS活性的诱导剂，脱落酸(ABA)对AOS活性无诱导作用。在类十八烷的水平上，不同诱导剂诱导AOS的结果明显不同，这取决于诱导剂的性质。伤害导致受伤叶片中茉莉酸的双相强烈积累，而系统叶片中茉莉酸水平的增加不那么明显。伤叶中OPDA含量变化不大，系统叶中OPDA含量基本不变甚至下降。乙烯利处理导致JA水平的强烈的、短暂的增加，与创伤诱导的JA的第二个更明显的峰值动力学上一致。在SA处理的叶片中，cis-OPDA水平在整个试验期内呈上升趋势，而JA水平在处理后24 h内没有变化，48 h后仅有少量积累。显然，除了调节底物(LA)的有效性和AOS的积累外，还控制着十八烷类物质途径的输出。
The analysis of allene oxide synthase (AOS) mRNA levels, of AOS polypeptide levels and specific enzymatic activities, as well as the quantitative determination of the levels of the octadecanoids cis-12-oxophytodienoic acid (cis-OPDA) and JA following a number of treatments, has shown that AOS is a regulatory site in octadecanoid biosynthesis in A. thaliana. AOS activity, mRNA and polypeptide levels are increased in wounded leaves locally and systemically. The methyl esters of OPDA or JA (OPDAME, JAME) and coronatine, are strong inducers of AOS mRNA, polypeptide and enzymatic activity. Ethephon also induces AOS activity. Salicylic acid (SA) was an inducer of AOS activity while abscisic acid (ABA) had no effect. At the level of the octadecanoids, the consequences of induction of AOS by the different inducers were distinctly different, depending on the nature of the inducer. Wounding led to a strong, bi-phasic accumulation of JA in wounded leaves and to a less pronounced increase in JA-levels in systemic leaves. Levels of OPDA changed very little in wounded leaves and remained constant or even declined in systemic leaves. Ethephon treatment resulted in a strong, transient increase in JA-levels kinetically coinciding with the second, more pronounced peak in wound-induced JA. In SA-treated leaves, the level of cis-OPDA increased throughout the experimental period while there was no effect on JA levels during the first 24 h following treatment and only a slight accumulation after 48 h. Clearly, mechanisms in addition to regulating substrate (LA) availability and the regulation of AOS accumulation control the output of the octadecanoid pathway.