Platinum-doped ceria based biosensor for in vitro and in vivo monitoring of lactate during hypoxia.
Platinum-doped ceria based biosensor for in vitro and in vivo monitoring of lactate during hypoxia.
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DOI：
10.1021/ac5047455
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发表时间：
2015-02
影响因子：
7.4
通讯作者：
Naimish P. Sardesai;Mallikarjunarao Ganesana;A. Karimi;J. Leiter;S. Andreescu
中科院分区：
文献类型：
--
作者：
Naimish P. Sardesai;Mallikarjunarao Ganesana;A. Karimi;J. Leiter;S. Andreescu
血液和组织中乳酸浓度的测量是组织氧合充分性的重要指示，并且可用于监测各种疾病的状态和进展。本文介绍了一种基于乳酸氧化酶和富氧铂掺杂二氧化铈（Pt-二氧化铈）纳米颗粒的电流型微生物传感器的制造，分析表征和生理应用，用于监测缺氧条件下的乳酸水平。Pt-二氧化铈纳米颗粒提供了用于检测酶促产生的过氧化氢的电催化放大，并充当酶的内部氧源，使得能够在缺氧组织中监测乳酸盐。在体外评价的生物传感器表现出高选择性对生理水平的抗坏血酸，3周的储存稳定性，6秒的快速响应时间，和良好的，线性的灵敏度在一个很宽的浓度范围内。通过将生物传感器放置在麻醉大鼠的海马中进行的体内实验证明了在2小时缺血和再灌注期间连续监测乳酸的可行性。结果表明，Pt-二氧化铈是一种通用的材料，用于植入式酶生物电极，可用于评估组织缺氧的病理生理学。除了在缺氧条件下的测量之外，该生物传感器的检测限很低，为100 pM，并且用于制造该生物传感器的材料在用于监测各种条件下的乳酸水平的超灵敏装置中特别有用。
Measurements of lactate concentrations in blood and tissues are an important indication of the adequacy of tissue oxygenation and could be useful for monitoring the state and progress of a variety of diseases. This paper describes the fabrication, analytical characterization, and physiological application of an amperometric microbiosensor based on lactate oxidase and oxygen-rich platinum doped ceria (Pt-ceria) nanoparticles for monitoring lactate levels during hypoxic conditions. The Pt-ceria nanoparticles provided electrocatalytic amplification for the detection of the enzymatically produced hydrogen peroxide and acted as an internal oxygen source for the enzyme, enabling lactate monitoring in an oxygen depleted tissue. In vitro evaluation of the biosensor demonstrated high selectivity against physiological levels of ascorbic acid, a storage stability of 3 weeks, a fast response time of 6 s, and good, linear sensitivity over a wide concentration range. In vivo experiments performed by placing the biosensor in the hippocampus of anesthetized rats demonstrated the feasibility of continuous lactate monitoring over 2 h ischemia and reperfusion. The results demonstrate that Pt-ceria is a versatile material for use in implantable enzyme bioelectrodes, which may be used to assess the pathophysiology of tissue hypoxia. In addition to measurements in hypoxic conditions, the detection limit of this biosensor was low, 100 pM, and the materials used to fabricate this biosensor can be particularly useful in ultrasensitive devices for monitoring lactate levels in a variety of conditions.