Inflammatory cytokine mRNA in human gestational tissues: Implications for term and preterm labor
Inflammatory cytokine mRNA in human gestational tissues: Implications for term and preterm labor
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DOI：
10.1016/s1071-5576(96)00042-1
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发表时间：
1996-11-01
期刊：
影响因子：
--
通讯作者：
Trautman, MS
中科院分区：
文献类型：
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作者：
Dudley, DJ;Collmer, D;Trautman, MS
目的：探讨炎性细胞因子在妊娠组织中的表达是否仅在感染相关的早产或其他临床条件下存在。方法：收集51例经足月无分娩剖宫产、2正常足月阴道分娩、3无分娩早产、4无绒毛膜羊膜炎的早产、5)早产合并绒毛膜羊膜炎的妊娠组织。分离蜕膜、绒毛和羊膜，提取各组织总RNA，聚合酶链式反应检测炎性细胞因子mRNA的表达。结果：IL-1β在未分娩的早产组织中很少表达，而在分娩后的母体和胎儿组织中均可检测到IL-1β的表达，且与胎龄无关。白介素6mRNA在非临产患者的组织中很少见，但在分娩后几乎所有组织中都能找到。IL-8mRNA在足月所有组织中均可检测到，无论是非临产患者还是临产患者。产后仅有20-50%的组织中检测到肿瘤坏死因子-α的表达，在无分娩的情况下很少检测到肿瘤坏死因子-α的表达。结论：炎性细胞因子在正常分娩和早产合并或不合并宫内感染后的妊娠组织中普遍表达。有或无临床明显宫内感染的妇女，其炎性细胞因子的表达模式无明显差异。版权所有(C)1996，由妇科调查学会提供。
OBJECTIVE: To determine if inflammatory cytokine mRNA in gestational tissues is present only in the setting of infection-associated preterm labor or under several other clinical conditions.METHODS: Human gestational tissues were collected from 51 women experiencing 1) term cesarean delivery without labor, 2) normal term vaginal delivery, 3) preterm cesarean delivery without labor, 4) preterm vaginal delivery without chorioamnionitis, and 5) preterm vaginal delivery with concomitant chorioamnionitis. Decidua, chorion, and amnion were isolate, total RNA from each tissue was extracted, and the presence of inflammatory cytokine mRNA was determined by polymerase chain reaction. Interleukin (IL)-1 beta, IL-6, IL-8, and tumor necrosis factor-alpha mRNA was detected using specific oligonucleotide primers.RESULTS: Interleukin-1 beta mRNA was rarely found in tissues preterm without labor but was readily detected in both maternal and fetal tissues after labor, regardless of gestational age. Interleukin-6 mRNA was rare in tissues from the nonlaboring patient but was found in almost all tissues after labor. Interleukin-8 mRNA was detected in all tissues at term, both in nonlaboring and laboring patients. Tumor necrosis factor-alpha mRNA was detected in only 20-50% of tissues after labor, and was rarely detected in the absence of labor.CONCLUSIONS: Inflammatory cytokine mRNA is commonly expressed in human gestational tissues after normal labor and preterm labor with or without associated intrauterine infection. There was no difference in the pattern of expression of mRNA inflammatory cytokine in women who did or did not hav clinically evident intrauterine infection. Copyright (C) 1996 by the Society for Gynecologic Investigation.