COMPARISON OF REGULATORY AND STRUCTURAL REGIONS OF THE XENOPUS-LAEVIS SMALL HEAT-SHOCK PROTEIN-ENCODING GENE FAMILY
COMPARISON OF REGULATORY AND STRUCTURAL REGIONS OF THE XENOPUS-LAEVIS SMALL HEAT-SHOCK PROTEIN-ENCODING GENE FAMILY
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DOI：
10.1016/0378-1119(92)90643-4
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发表时间：
1992-01-15
期刊：
影响因子：
3.5
通讯作者：
HEIKKILA, JJ
中科院分区：
文献类型：
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作者：
KRONE, PH;SNOW, A;HEIKKILA, JJ
我们已经分离出几个独特的非洲爪蟾hsp 30（编码热休克蛋白30）基因组克隆，其中一个包含两个完整的hsp 30基因（hsp 30 C和hsp 30 D），以及启动子和N-末端编码区的第三个基因（hsp 30 E）。 核苷酸序列和限制性内切酶分析表明，该基因簇与以前分离的基因簇不同。 hsp 30 C和hsp 30 D基因编码约100个氨基酸的蛋白质。24 kDa。 总的来说，hsp 30基因家族至少包含7个基因。 产生该基因家族的链交换和复制事件的断裂似乎发生在可能呈现Z-DNA构象的DNA片段内。 比较每个已知的Hsp 30蛋白质的氨基酸（aa）序列与牛α-晶状体蛋白揭示了高度共享的保守性的aa构成的主要结构特征（S）的α-晶状体蛋白。
We have isolated several unique Xenopus laevis hsp30 (encoding heat-shock protein 30) genomic clones, one of which contains two complete hsp30 genes (hsp30C and hsp30D), as well as the promoter and N-terminal coding region of a third gene (hsp30E). Nucleotide sequence and restriction enzyme analysis revealed that this gene cluster is different from a cluster isolated previously. The hsp30C and hsp30D genes encode proteins of approx. 24 kDa. In all, the hsp30 gene family contains a minimum of seven genes. The strand exchange and breakage of the duplication events which generated this gene family appear to have occurred within tracts of DNA which potentially can assume a Z-DNA conformation. Comparing the amino acid (aa) sequences of each known Hsp30 protein with bovine alpha-crystallin revealed a high degree of shared conservation of aa that constitute the major structural feature(s) of alpha-crystallin.