Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants.
Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants.
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DOI：
10.1007/7651_2017_13
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发表时间：
2018
影响因子：
--
通讯作者：
Kentaro Furukawa;T. Kanki
中科院分区：
文献类型：
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作者：
Kentaro Furukawa;T. Kanki
线粒体自噬(有丝分裂吞噬)是通过自噬选择性降解线粒体的过程。最近的研究表明，有丝分裂通过降解受损或过剩的线粒体在线粒体动态平衡中发挥重要作用。芽殖酵母酿酒酵母是一种功能强大的模式生物，已被用于研究多种生物现象。最近，在鉴定了线粒体外膜受体蛋白Atg32之后，对酵母中吞丝分裂的理解有了很大的进展。在这一章中，我们描述了利用荧光显微镜和免疫印迹技术，通过全基因组筛选有丝分裂缺陷突变体来研究酵母中有丝分裂的方法。
Mitochondrial autophagy (mitophagy) is a process that selectively degrades mitochondria via autophagy. Recent studies have shown that mitophagy plays an important role in mitochondrial homeostasis by degrading damaged or excess mitochondria. The budding yeastSaccharomyces cerevisiaeis a powerful model organism that has been employed to study several biological phenomena. Recently, there has been significant progress in the understanding of mitophagy in yeast following the identification of Atg32, a mitochondrial outer membrane receptor protein for mitophagy. In this chapter, we describe protocols to study mitophagy in yeast via a genome-wide screen for mitophagy-deficient mutants using fluorescence microscopy and immunoblotting.