Development of an affinity-driven cross-linker: isolation of a vitamin D receptor associated factor.
Development of an affinity-driven cross-linker: isolation of a vitamin D receptor associated factor.
复制标题
亲和力驱动交联剂的开发：维生素 D 受体相关因子的分离。
DOI：
10.1016/s0960-894x(00)00009-3
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发表时间：
2000
影响因子：
2.7
通讯作者：
Ray,R
中科院分区：
文献类型：
--
作者：
Swamy,N;Addo,JK;Ray,R
合成了含有亲和性和光亲和性探针（亲和性驱动的交联剂，双标记）的维生素D类似物。利用GST-VDR-配体结合域融合蛋白（GST-VDR-LBD）、双标记亲和标记和蛋白质-蛋白质交联技术，从大鼠肝细胞核提取物中分离得到一种与维生素D受体（VDR）相关的未知因子。
A vitamin D analogue containing an affinity and a photoaffinity probe (affinity-driven cross-linker, Double Label) was synthesized. An unknown factor, associated with vitamin D receptor (VDR), was isolated from rat liver nuclear extract using a GST-VDR-ligand-binding domain fusion protein (GST-VDR-LBD), affinity labeled with Double Label, and protein–protein cross-linking by photolysis.