Glycosaminoglycans produced in tissue culture by rat lung cells. Isolation from a mixed cell line and a derived endothelial clone.
Glycosaminoglycans produced in tissue culture by rat lung cells. Isolation from a mixed cell line and a derived endothelial clone.
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大鼠肺细胞在组织培养中产生的糖胺聚糖。
DOI：
10.3109/03008207509152196
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发表时间：
1975
影响因子：
2.9
通讯作者：
G. Turino
中科院分区：
文献类型：
--
作者：
P. Sampson;M. Parshley;I. Mandl;G. Turino
分离并检测了正常成年大鼠肺混合细胞系和来源于该细胞系的内皮细胞克隆产生的糖胺聚糖。用特定的酶消化前后的培养基和细胞的醋酸纤维素电泳表明，除硫酸角质素外，所有主要的糖胺聚糖都是由两种培养物合成的。硫酸乙酰肝素和硫酸皮肤素仅在细胞组分中发现，而透明质酸在培养基和细胞组分中均发现。从培养基中分离硫酸软骨素。内皮细胞克隆产生的葡萄糖胺半乳糖胺在培养基中的比例为4：1，从第五至第十三个月的文化。混合细胞系的培养基最初含有葡糖胺与半乳糖胺比率为2：1的葡糖胺聚糖，但培养约一年后，该比率变为4.6：1，表明培养物主要含有内皮细胞。
The glycosaminoglycans produced by a mixed cell line of normal adult rat lung and an endothelial clone derived from this line were isolated and examined. Cellulose acetate electrophoresis of media and cells before and after digestion with specific enzymes indicated that all the major glycosaminoglycans except keratan sulfate were synthesized by both cultures. Heparan sulfate and dermatan sulfate were found only in the cell fraction while hyaluronic acid was found in both the medium and the cell fractions. The chondroitin sulfates were isolated from the medium. The endothelial clone produced a 4:1 ratio of glucosamine to galactosamine in the medium from the fifth through thirteenth months of culture. The medium of the mixed cell line initially contained glycosaminoglycans with a glucosamine to galactosamine ratio of 2:1 but after approximately one year of culture, the ratio had changed to 4.6:1 suggesting that the culture contained predominatly endothelial cells.