Simultaneous dual-color 3D STED microscopy
Simultaneous dual-color 3D STED microscopy
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DOI：
10.1364/oe.22.007028
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发表时间：
2014-03-24
期刊：
影响因子：
3.8
通讯作者：
Michaelis, Jens
中科院分区：
文献类型：
--
作者：
Osseforth, Christian;Moffitt, Jeffrey R.;Michaelis, Jens
我们描述了一种受激发射损耗（STED）显微镜的设计和实现，该显微镜允许同时在两种颜色中进行三维超分辨率成像。使用超连续激光源提供激发和有效耗尽所需的所有光谱带，以实现分别接近35 nm和90 nm的横向和轴向分辨率。我们通过成像胶体粒子和单荧光分子来表征系统的性能。在哺乳动物细胞中核孔复合物和DNA复制位点的双色成像证明了它的生物学适用性。(C)2014年美国光学学会
We describe the design and implementation of a stimulated emission depletion (STED) microscope which allows simultaneous three-dimensional super-resolution imaging in two colors. A super-continuum laser source is used to provide all spectral bands necessary for excitation and efficient depletion to achieve a lateral and axial resolution of similar to 35 nm and similar to 90 nm respectively. We characterize the systems' performance by imaging colloidal particles and single fluorescent molecules. Its biological applicability is demonstrated by dual-color imaging of nuclear pore complexes and of DNA replication sites in mammalian cells. (C)2014 Optical Society of America