Nanoparticles Meet Cell Membranes: Probing Nonspecific Interactions. using Model Membranes
Nanoparticles Meet Cell Membranes: Probing Nonspecific Interactions. using Model Membranes
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DOI：
10.1021/es403864v
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发表时间：
2014-01-21
影响因子：
11.4
通讯作者：
Bothun, Geoffrey D.
中科院分区：
文献类型：
--
作者：
Chen, Kai Loon;Bothun, Geoffrey D.
纳米毒性研究表明，碳基和无机工程纳米颗粒都可能对微生物有毒。虽然细胞毒性的途径多种多样，并且取决于工程纳米颗粒的性质和化学环境，但大量研究提供的证据表明，纳米颗粒和细菌细胞膜之间的直接接触对于细胞失活或损伤是必要的，并且实际上可能是细胞毒性的主要机制。由于生物膜的异质性和动态性质，纳米粒子附着和破坏细胞膜的倾向仍然没有得到很好的了解。模型生物膜可用于纳米颗粒-膜相互作用的系统研究。在本文中，将讨论当前和新兴的实验方法，以确定控制 ENP 在模型膜上附着和 ENP 对膜的破坏的关键参数。这一关键信息将有助于实现无毒或生物相容的工程纳米颗粒的“安全设计”生产，并且还允许设计用于环境和生物医学应用的抗菌纳米颗粒。
Nanotoxicity studies have shown that both carbon-based and inorganic engineered nanoparticles can be toxic to microorganisms. While the pathways for cytotoxicity are diverse and dependent upon the nature of the engineered nanoparticle and the chemical environment, numerous studies have provided evidence that direct contact between nanoparticles and bacterial cell membranes is necessary for cell inactivation or damage, and may in fact be a primary mechanism for cytotoxicity. The propensities for nanoparticles to attach to and disrupt cell membranes are still not well understood due to the heterogeneous and dynamic nature of biological membranes. Model biological membranes can be employed for systematic investigations of nanoparticle−membrane interactions. In this article, current and emerging experimental approaches to identify the key parameters that control the attachment of ENPs on model membranes and the disruption of membranes by ENPs will be discussed. This critical information will help enable the “safe-by-design” production of engineered nanoparticles that are nontoxic or biocompatible, and also allow for the design of antimicrobial nanoparticles for environmental and biomedical applications.