Direct and indirect targets of the arabidopsis seed transcription factor ABSCISIC ACID INSENSITIVE3
Direct and indirect targets of the arabidopsis seed transcription factor ABSCISIC ACID INSENSITIVE3
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DOI：
10.1111/tpj.14854
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发表时间：
2020-06-22
期刊：
影响因子：
7.2
通讯作者：
Perry, Sharyn E.
中科院分区：
文献类型：
--
作者：
Tian, Ran;Wang, Fangfang;Perry, Sharyn E.
拟南芥脱落酸不敏感3（Abscisic Acid Insensitive 3，ABI 3）是B3结构域家族中的一个转录因子。ABI 3与B3结构域转录因子LEAFY COTYLEDON 2（LEC 2）和FUSCA 3（FUS 3）以及LEC 1（CCAAT盒结合复合物的亚基）一起，沿着形成所谓的LAFL网络，以控制种子发育和成熟的各个方面。ABI 3也有助于脱落酸（阿坝）反应。我们报告染色质免疫沉淀平铺阵列实验地图结合位点ABI 3全球。我们还通过比较发育中的abi 3 - 5和野生型种子来评估对ABI 3应答的转录组，并结合这些信息来确定直接和间接应答的ABI 3靶基因。ABI 3能直接诱导和抑制靶基因的转录，并且这两组基因之间存在一些有趣的差异。直接调节的靶标反映了ABI 3在种子成熟、脱水耐受性、进入静止状态和寿命中的作用。有趣的是，ABI 3直接抑制一个编码microRNA（MIR 160 B）的基因，该基因靶向参与休眠建立的生长素反应因子（ARF）10和ARF 16。此外，ABI 3与FUS 3一样，调节编码MIR 156的基因，但FUS 3仅诱导编码该产物的基因，而ABI 3在种子发育的早期阶段诱导这些基因，但在发育后期抑制这些基因。ABI 3、其他LAFL基因和参与向幼苗发育过渡的VP 1/ABI 3-LIKE（瓦尔）基因之间的相互作用被检查，并揭示了控制发育的复杂相互作用。
Arabidopsis thalianaABSCISIC ACID INSENSITIVE3 (ABI3) is a transcription factor in the B3 domain family. ABI3, along with B3 domain transcription factors LEAFY COTYLEDON2 (LEC2) and FUSCA3 (FUS3), and LEC1, a subunit of the CCAAT box-binding complex, form the so-called LAFL network to control various aspects of seed development and maturation. ABI3 also contributes to the abscisic acid (ABA) response. We report on chromatin immunoprecipitation-tiling array experiments to map binding sites for ABI3 globally. We also assessed transcriptomes in response to ABI3 by comparing developingabi3-5and wild-type seeds and combined this information to ascertain direct and indirect responsive ABI3 target genes. ABI3 can induce and repress its transcription of target genes directly and some intriguing differences exist incismotifs between these groups of genes. Directly regulated targets reflect the role of ABI3 in seed maturation, desiccation tolerance, entry into a quiescent state and longevity. Interestingly, ABI3 directly represses a gene encoding a microRNA (MIR160B) that targetsAUXIN RESPONSE FACTOR(ARF)10andARF16that are involved in establishment of dormancy. In addition, ABI3, like FUS3, regulates genes encodingMIR156but while FUS3 only induces genes encoding this product, ABI3 induces these genes during the early stages of seed development, but represses these genes during late development. The interplay between ABI3, the otherLAFLgenes, and theVP1/ABI3-LIKE(VAL) genes, which are involved in the transition to seedling development are examined and reveal complex interactions controlling development.