Photoreactivity of 5-iodouracil-containing DNA-Sso7d complex.
Photoreactivity of 5-iodouracil-containing DNA-Sso7d complex.
复制标题
含有 5-碘尿嘧啶的 DNA-Sso7d 复合物的光反应性。
DOI：
10.1093/nass/42.1.171
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
A. Wang
中科院分区：
文献类型：
--
作者：
T. Oyoshi;H. Sugiyama;A. Wang
DNA-Sso 7 d复合物的X-射线结构表明，该蛋白的结合引起急剧的DNA弯曲。为了研究该蛋白质是否也导致溶液中的DNA弯曲，研究了在Sso 7 d蛋白质存在和不存在的情况下1U-取代的DNA的光反应。结果发现，在观察到的晶体结构的弯曲部位，在相邻胸腺嘧啶的甲基处有效地发生了不寻常的链内氢提取。
X-ray structure of DNA-Sso7d complex indicated that binding of this protein causes sharp DNA bending. In order to examine whether this protein also causes DNA bending in solution, photoreactions of 1U-substituted DNA in the presence and the absence of Sso7d protein were investigated. It was found that the unusual intrastrand hydrogen abstraction at methyl of adjacent thymine occurred efficiently at the observed bending site of crystal structure.