MESENCHYMAL STEM CELL THERAPY FOR CUTANEOUS RADIATION SYNDROME
MESENCHYMAL STEM CELL THERAPY FOR CUTANEOUS RADIATION SYNDROME
复制标题
DOI：
10.1097/hp.0b013e3181d3d52c
复制
发表时间：
2010-06-01
期刊：
影响因子：
2.2
通讯作者：
Yamashita, Shunichi
中科院分区：
文献类型：
--
作者：
Akita, Sadanori;Akino, Kozo;Yamashita, Shunichi
应预防或适当治疗核电反应堆事故、治疗性照射或核恐怖主义造成的全身和局部辐射损伤，以改善伤口管理并挽救生命。目前，再生手术方式应尝试颞人工真皮浸渍和喷涂局部血管生成因子，如碱性成纤维细胞生长因子，和二次重建可以是一个候选人的分界和保存供体发病率。将人间充质干细胞和脂肪源性干细胞与血管生成和促有丝分裂因子碱性成纤维细胞生长因子和人工真皮一起应用于切除的辐射皮肤缺损，并测试辐射暴露伤口中的分化和局部刺激作用。对于有复杂基础疾病的患者，穿支皮瓣和含生长因子的人工真皮模板均能成功修复。患者均顺利接受治疗，发病率极低。在实验中，即使在体外20戈伊照射后，hMSCs仍具有强烈的增殖能力。在体内，4戈伊大鼠全身照射证实了持续的骨髓基质（间充质干）细胞在骨髓中存活。在20戈伊照射的皮肤和软组织上立即应用浸渍有细胞和细胞因子的人工真皮证明了显著改善的脂肪血管生成、构建的真皮重建和较少的炎性表皮恢复。详细了解基础疾病和合理的重建程序带来了很好的结果难以照射伤口愈合。脂肪来源的干细胞还涉及同一受试者中短细胞收获和处理时间的有限局部损伤。健康物理98（6）：858-862; 2010
Systemic and local radiation injuries caused by nuclear power reactor accidents, therapeutic irradiation, or nuclear terrorism should be prevented or properly treated in order to improve wound management and save lives. Currently, regenerative surgical modalities should be attempted with temporal artificial dermis impregnated and sprayed with a local angiogenic factor such as basic fibroblast growth factor, and secondary reconstruction can be a candidate for demarcation and saving the donor morbidity. Human mesenchymal stem cells and adipose-derived stem cells, together with angiogenic and mitogenic factor of basic fibroblast growth factor and an artificial dermis, were applied over the excised irradiated skin defect and were tested for differentiation and local stimulation effects in the radiation-exposed wounds. The perforator flap and artificial dermal template with growth factor were successful for reconstruction in patients who were suffering from complex underlying disease. Patients were uneventfully treated with minimal morbidities. In the experiments, the hMSCs are strongly proliferative even after 20 Gy irradiation in vitro. In vivo, 4 Gy rat whole body irradiation demonstrated that sustained marrow stromal (mesenchymal stem) cells survived in the bone marrow. Immediate artificial dermis application impregnated with cells and the cytokine over the 20 Gy irradiated skin and soft tissues demonstrated the significantly improved fat angiogenesis, architected dermal reconstitution, and less inflammatory epidermal recovery. Detailed understanding of underlying diseases and rational reconstructive procedures brings about good outcomes for difficult irradiated wound healing. Adipose-derived stem cells are also implicated in the limited local injuries for short cell harvesting and processing time in the same subject. Health Phys. 98(6): 858-862; 2010