SYNERGY DURING IN VITRO CYTOTOXIC ALLOGRAFT RESPONSES
SYNERGY DURING IN VITRO CYTOTOXIC ALLOGRAFT RESPONSES
复制标题
体外细胞毒性同种异体移植反应期间的协同作用
DOI：
--
复制
发表时间：
1973
影响因子：
15.3
通讯作者：
H. Wagner
中科院分区：
文献类型：
--
作者：
H. Wagner
用小鼠体外同种异体移植系统评价T细胞介导的细胞免疫中T-T相互作用的概念。在分析来自不同组织的经胸腺处理的淋巴细胞的反应性时，发现T细胞在体外对移植抗原的免疫能力具有遗传性。循环T细胞是胸腺细胞的10-20倍，脾T细胞居中。当少数(1.5×106)外周T细胞数量太少而不能产生良好的细胞毒反应时，将其与14×106胸腺细胞混合，并在体外免疫细胞结合的同种异体抗原，产生的细胞毒活性约为纯相加效应所能解释的值的10-20倍。来自CBA(H-2k)和AKR(H-2k)小鼠的应答T细胞的混合物中也出现了协同作用。因此，AKR抗φC3H血清可用于胸腺来源和外周血T细胞来源的细胞毒淋巴细胞(CL)的鉴别。胸腺细胞与外周T细胞协同作用产生的细胞毒活性约70%来自T细胞，其余来自胸腺。对这一发现的天气学解释和反应细胞的“极限稀释”实验有力地表明，外周T细胞是CL前体细胞的主要来源，胸腺细胞主要起辅助(放大)细胞的作用。
A mouse in vitro allograft system was used to evaluate the concept of T-T interaction in T cell-mediated cellular immunity. In analyzing the responsiveness of thymus-processed lymphocytes as obtained from different tissues, a heretogeneity within T cells was found in regard to their capacity to be immunized in vitro against transplantation antigens. Recirculating T cells were 10–20-fold superior to thymocytes, splenic T cells being intermediate. When few (1.5 x 106) peripheral T cells, in numbers too small to yield good cytotoxic responses, were mixed with 14 x 106 thymocytes and the cell mixture immunized in vitro against cell-bound alloantigens, cytotoxic activity was generated exceeding about 10–20-fold the values that could be explained by a pure additive effect. Synergy occurred also in a mixture of responder T cells derived from CBA (H-2k) and AKR (H-2k) mice. Thus AKR anti-φ C3H serum could be used for discriminating between thymus-derived and peripheral T cell-derived cytotoxic lymphocytes (CL). Cytotoxic activity produced during the synergistic interaction between thymocytes and peripheral T cells was about 70% T cell derived, the remainder being thymus derived. The synoptic interpretation of this finding and "limiting dilution" experiments of the responder cells suggested strongly that peripheral T cells provide the major source for precursor cells of CL, thymocytes acting mainly as helper (amplifier) cells.