PRODUCTION OF INFECTIOUS POLIOVIRUS FROM CLONED CDNA IS DRAMATICALLY INCREASED BY SV40 TRANSCRIPTION AND REPLICATION SIGNALS
PRODUCTION OF INFECTIOUS POLIOVIRUS FROM CLONED CDNA IS DRAMATICALLY INCREASED BY SV40 TRANSCRIPTION AND REPLICATION SIGNALS
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DOI：
10.1093/nar/12.12.5123
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
WIMMER, E
中科院分区：
文献类型：
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作者：
SEMLER, BL;DORNER, AJ;WIMMER, E
代表脊髓灰质炎病毒1型（Mahoney）整个基因组RNA的亚基因组cDNA克隆已在大肠杆菌中分离。从这些克隆中，在质粒载体pBR 325的EcoRI位点构建脊髓灰质炎病毒基因组的完整cDNA拷贝。通过转染将含有脊髓灰质炎RNA的完整cDNA拷贝的质粒DNA引入培养的灵长类动物细胞中产生感染性脊髓灰质炎病毒。通过这种转染产生的病毒似乎与野生型脊髓灰质炎病毒相同。还描述了含有SV 40转录和复制信号的脊髓灰质炎重组质粒的分离。用该质粒转染COS-1细胞，每μ g输入DNA产生大于1，600噬斑形成单位（PFU）。
Sub-genomic cDNA clones representing the entire genomic RNA of poliovirus Type 1 (Mahoney) have been isolated inE.coli. Construction of a complete cDNA copy of the poliovirus genome in theEcoRI site of plasm id vector pBR325 from these clones is described. Introduction of plasmid DNA containing the complete cDNA copy of polio RNA into cultured primate cells by transfection produces infectious poliovirus. The virus produced by such a transfection appears to be identical to wild type poliovirus. Isolation of a polio recombinant plasmid containing SV40 transcription and replication signals is also described. Transfection of COS-1 cells with this plasmid yields greater than 1,600 plaque-forming units (PFU) per ug of input DNA.