AN ANCIENT, HIGHLY CONSERVED FAMILY OF CYSTEINE-RICH PROTEIN DOMAINS REVEALED BY CLONING TYPE-I AND TYPE-II MURINE MACROPHAGE SCAVENGER RECEPTORS
AN ANCIENT, HIGHLY CONSERVED FAMILY OF CYSTEINE-RICH PROTEIN DOMAINS REVEALED BY CLONING TYPE-I AND TYPE-II MURINE MACROPHAGE SCAVENGER RECEPTORS
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DOI：
10.1073/pnas.87.22.8810
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发表时间：
1990-11-01
影响因子：
11.1
通讯作者：
KRIEGER, M
中科院分区：
文献类型：
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作者：
FREEMAN, M;ASHKENAS, J;KRIEGER, M
清道夫受体与动脉粥样硬化和其他巨噬细胞相关功能的发展有关。牛I型和II型清道夫受体是多结构域跨膜蛋白，它们的区别仅在于I型受体存在一个额外的细胞外富含半胱氨酸的c端结构域。从小鼠巨噬细胞系p388D1中分离出I型和II型受体cdna，确定了在单个细胞中存在编码两种受体类型的mrna。它们的序列与牛的cdna高度相似。受体类型特异性cDNA探针定位到小鼠8号染色体上的一个共同位点，表明一个基因编码这两种mrna。i型特异性清除率受体富半胱氨酸（SRCR）结构域有助于定义一个以前未被识别的非常保守的结构域家族。高度同源的SRCR结构域（每个多肽链有1、3或4个）存在于人类（如CD5、补体因子I）、小鼠（Ly-1）和海胆（精子受体）的多种分泌蛋白和细胞表面蛋白中。
Scavenger receptors have been implicated in the development of atherosclerosis and other macrophage-associated functions. The bovine type I and type II scavenger receptors are multidomain transmembrane proteins that differ only by the presence in the type I receptor of an additional, extracellular cysteine-rich C-terminal domain. The isolation of type I and type II receptor cDNAs from a murine macrophage cell line, p388D1, establishes the presence of mRNAs encoding both receptor types in a single cell. Their sequences are highly similar to the bovine cDNAs. Receptor type-specific cDNA probes map to a common locus on murine chromosome 8, suggesting that a single gene encodes both mRNAs. The type I-specific scavenger receptor cysteine-rich (SRCR) domain helps define a previously unrecognized family of remarkably well-conserved domains. Highly homologous SRCR domains (one, three, or four per polypeptide chain) are found in diverse secreted and cell-surface proteins from humans (e.g., CD5, complement factor I), mice (Ly-1), and sea urchins (speract receptor).