Genetic analysis of the GLUT10 glucose transporter (SLC2A10) polymorphisms in Caucasian American type 2 diabetes -: art. no. 42
Genetic analysis of the GLUT10 glucose transporter (SLC2A10) polymorphisms in Caucasian American type 2 diabetes -: art. no. 42
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DOI：
10.1186/1471-2350-6-42
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发表时间：
2005-12-07
影响因子：
--
通讯作者：
Segade, F
中科院分区：
文献类型：
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作者：
Bento, JL;Bowden, DW;Segade, F
背景资料：GLUT 10（基因符号SLC 2A 10）是位于染色体20 q12 -13.1上的2型糖尿病（T2 DM）相关区域内的促进性葡萄糖转运蛋白。方法：采用Sequenom MassArray基因分型系统，对300例T2 DM患者和310例对照者的SLC 2A 10基因的100 kb区域内的20个SNPs（包括4个编码区、10个内含子区、6个5'和3'区）进行基因分型。分析SLC 2A 10的等位基因关联性、连锁不平衡（LD）和单倍型结构，以确定是否存在与2型糖尿病相关的单倍型。结果：15个变异体的杂合度大于0.80，并进一步分析其与2型糖尿病的关联性。未观察到任何变异与T2 DM显著相关的证据（所有P = 0.05），包括Ala 206 Thr（rs 2235491），先前报告其与空腹胰岛素相关。连锁不平衡分析表明，SLC 2A 10基因包含在一个14 kb的单倍型块。单倍型关联分析与T2 DM没有发现任何显着差异单倍型频率在T2 DM病例和controls.Conclusion：从我们的研究结果，我们可以得出结论，序列变异或附近的GLUT 10是不太可能作出显着贡献的T2 DM在高加索美国人。
Background: GLUT10 (gene symbol SLC2A10) is a facilitative glucose transporter within the type 2 diabetes (T2DM)-linked region on chromosome 20q12-13.1. Therefore, we evaluated GLUT10 as a positional candidate gene for T2DM in Caucasian Americans.Methods: Twenty SNPs including 4 coding, 10 intronic and 6 5' and 3' to the coding sequence were genotyped across a 100 kb region containing the SLC2A10 gene in DNAs from 300 T2DM cases and 310 controls using the Sequenom MassArray Genotyping System. Allelic association was evaluated, and linkage disequilibrium (LD) and haplotype structure of SLC2A10 were also determined to assess whether any specific haplotypes were associated with T2DM.Results: Of these variants, fifteen had heterozygosities greater than 0.80 and were analyzed further for association with T2DM. No evidence of significant association was observed for any variant with T2DM (all P = 0.05), including Ala206Thr (rs2235491) which was previously reported to be associated with fasting insulin. Linkage disequilibrium analysis suggests that the SLC2A10 gene is contained in a single haplotype block of 14 kb. Haplotype association analysis with T2DM did not reveal any significant differences between haplotype frequencies in T2DM cases and controls.Conclusion: From our findings, we can conclude that sequence variants in or near GLUT10 are unlikely to contribute significantly to T2DM in Caucasian Americans.