DisAp-dependent striated fiber elongation is required to organize ciliary arrays
DisAp-dependent striated fiber elongation is required to organize ciliary arrays
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DOI：
10.1083/jcb.201409123
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发表时间：
2014-12-22
影响因子：
7.8
通讯作者：
Pearson, Chad G.
中科院分区：
文献类型：
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作者：
Galati, Domenico F.;Bonney, Stephanie;Pearson, Chad G.
纤毛组织基体（BB）是微管支架，它们明显不对称，因为它们有附加的辅助结构，如条纹纤维。在多纤毛细胞中，BB方向对齐以确保连贯的纤毛跳动，但维持BB方向的机制尚不清楚。对于第一次在四膜虫嗜热，我们使用比较全基因组测序，以确定在BB定向障碍突变disA-1的突变。disA-1消除了新蛋白DisAp对T的定位。这与DisAp与条纹纤维蛋白SF-组装蛋白的相似性一致。我们证明，DisAp是KFs伸长和抵抗BB定向障碍所需的纤毛力。当新形成的BB接近它们的皮质附着位点时，它们沿着KF移动。然而，因为它们含有旋转的短KFs，disA-1细胞中的BB显示异常的间距和定向障碍。因此，DisAp是一种新型KF组分，对于多纤毛阵列中的力依赖性KF伸长和BB取向至关重要。
Cilia-organizing basal bodies (BBs) are microtubule scaffolds that are visibly asymmetrical because they have attached auxiliary structures, such as striated fibers. In multiciliated cells, BB orientation aligns to ensure coherent ciliary beating, but the mechanisms that maintain BB orientation are unclear. For the first time in Tetrahymena thermophila, we use comparative whole-genome sequencing to identify the mutation in the BB disorientation mutant disA-1. disA-1 abolishes the localization of the novel protein DisAp to T. thermophila striated fibers (kinetodesmal fibers; KFs), which is consistent with DisAp's similarity to the striated fiber protein SF-assemblin. We demonstrate that DisAp is required for KFs to elongate and to resist BB disorientation in response to ciliary forces. Newly formed BBs move along KFs as they approach their cortical attachment sites. However, because they contain short KFs that are rotated, BBs in disA-1 cells display aberrant spacing and disorientation. Therefore, DisAp is a novel KF component that is essential for force-dependent KF elongation and BB orientation in multiciliary arrays.