Detection of micrometastatic prostate cancer cells in lymph nodes by reverse transcriptase-polymerase chain reaction.
Detection of micrometastatic prostate cancer cells in lymph nodes by reverse transcriptase-polymerase chain reaction.
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通过逆转录聚合酶链反应检测淋巴结中的微转移前列腺癌细胞。
作者：
Takashi Deguchi;Tatsuo Doi;Hideo Ehara;Shinichi Ito;Yoshito Takahashi;Y. Nishino;Shigeru Fujihiro;Takeshi Kawamura;H. Komeda;Masanori Horie;Hideki Kaji;Kuniyasu Shimokawa;Takuji Tanaka;Yukimichi Kawada
我们利用前列腺特异性抗原基因的特异性引物，建立了一种检测前列腺癌细胞的高灵敏度方法--逆转录聚合酶链式反应。应用逆转录聚合酶链式反应(RT-PCR)检测22例前列腺癌患者的44个淋巴结。逆转录聚合酶链式反应可在5个组织学和/或免疫组织化学阳性的淋巴结和4个组织学和免疫组织化学阴性的淋巴结中检测到前列腺特异性抗原mRNA。逆转录聚合酶链式反应是一种较组织学和免疫组织化学更敏感的检测前列腺癌转移细胞的方法，可用于前列腺癌淋巴结微转移的诊断。这种高度敏感的RT-PCR将是一种相关的工具，可以更准确地临床评估前列腺癌的淋巴转移，并从生物学上了解前列腺癌的淋巴扩散。
We have developed a highly sensitive method for detecting prostate cancer cells using reverse transcriptase-polymerase chain reaction (RT-PCR) with primers specific for prostate-specific antigen gene. Forty-four lymph nodes obtained from 22 patients with prostate cancers were analyzed by RT-PCR to detect metastatic prostate cancer cells. RT-PCR could detect prostate-specific antigen mRNA in five lymph nodes with histologically and/or immunohistochemically identifiable metastases and in four lymph nodes with negative histological and immunohistochemical analyses for metastases. RT-PCR was a more sensitive method than histology and immunohistochemistry in detecting metastatic prostate cancer cells and could be applied for diagnosing micrometastases of prostate cancer to lymph nodes. This highly sensitive RT-PCR will be a relevant tool to allow a more accurate clinical assessment of lymph node metastases of prostate cancer and to understand lymphatic dissemination of prostate cancer biologically.