OMIP-041: Optimized multicolor immunofluorescence panel rat microglial staining protocol
OMIP-041: Optimized multicolor immunofluorescence panel rat microglial staining protocol
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DOI：
10.1002/cyto.a.23267
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发表时间：
2018-02-01
期刊：
影响因子：
3.7
通讯作者：
Olson, Scott D.
中科院分区：
文献类型：
--
作者：
Furman, Naama E. Toledano;Prabhakara, Karthik S.;Olson, Scott D.
BACKGROUNDThe Optimized Multicolor Immunofluorescence Panel (OMIP) was designed to phenotypically characterize rat-derived microglial cells isolated from brain or spinal cord, in TBI or SCI rat models. Central nervous system (CNS) injury immediately results in a robust inflammatory cell-mediated response. The key players in the response to the injury are microglial cells, which are a major cellular component of the CNS innate immune system. In normal situations, microglia maintains a dynamic and ramified phenotype, while an injury results in a dramatic amoeboid phenotype change. CNS injuries also lead to infiltration of macrophages to the injury site and both microglia and macrophages share the expression of surface markers such as CD45, CD11b/c, and CX3CR1. The common way of identifying microglia
