Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population
Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population
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DOI：
10.1051/forest:2008055
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发表时间：
2008-10-01
影响因子：
3
通讯作者：
El-Kassaby, Yousry A.
中科院分区：
文献类型：
--
作者：
Funda, Tomas;Chen, Charles C.;El-Kassaby, Yousry A.
研究了西部落叶松（Larix occurentalis Nutt.）利用微卫星标记和父系-交配系统分析相结合的方法对实验群体进行了研究。通常难以评估，雄性配子的贡献，确定了95%的置信度，其对遗传增益和多样性的影响进行了测定。雄性生育成功率范围为0和11%之间。雄性有效群体大小与雄性有效群体大小之间存在显著差异，雄性有效群体大小与雄性有效群体大小之间存在显著差异，雄性有效群体大小与雄性有效群体大小之间存在显著差异，雄性有效群体大小与雄性有效群体大小之间存在显著差异，雄性有效群体大小与雄性有效群体大小之间存在显著差异（遗传多样性）估计从父权分配相比，那些根据人口普查数字结果表明，在14个种子园中，共筛选出221个全家系（样本量为1-8），并将其嵌套在种子园中，采用父系-交配系统相结合的方法分析种子园交配动态。虽然花粉流往往会增加交配系统的异交率，但亲子分析有效地确定了接受雌性的污染率和程度。
The mating pattern and gene flow in a western larch (Larix occidentalis Nutt.) experimental population was studied with the aid of microsatellite markers and a combination of paternity-mating system analysis. The commonly difficult to assess, male gametic contribution was determined with 95% confidence and its impact on genetic gain and diversity was determined.Male fertility success rate ranged between 0 and 11%. Male reproductive output parental imbalance was observed with 50% of the pollen being produced by the top 5% of males while the lower 39% males only produced 10% of the pollen.A significant difference was observed between male effective population size (genetic diversity) estimates from paternity assignment compared to those based on population's census number (21 vs. 41); however, this difference did not affect estimates of genetic gain.A total of 221 full-fib families were identified (sample size range: 1-8) and were nested among the studied 14 seed-donors.A combination of paternity-mating system analysis is recommended to provide a better insight into seed orchards' mating dynamics. While pollen flow tends to inflate mating system's outcrossing rate, the paternity analysis effectively determined the rate and magnitude of contamination across receptive females.