Mapping of five new putative anion transporter genes in human and characterization of SLC26A6, a candidate gene for pancreatic anion exchanger
Mapping of five new putative anion transporter genes in human and characterization of SLC26A6, a candidate gene for pancreatic anion exchanger
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DOI：
10.1006/geno.2000.6355
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发表时间：
2000-11-15
期刊：
影响因子：
4.4
通讯作者：
Kere, J
中科院分区：
文献类型：
--
作者：
Lohi, H;Kujala, M;Kere, J
除了经典的SLC 4（或AE）家族外，最近还描述了第二个不同的阴离子转运蛋白家族。SLC 26家族的成员在结构上非常保守，并且可以像SLC 4家族的成员一样介导Cl-与HCO 3-跨哺乳动物细胞质膜的电中性交换。SLC 26 A2（DTDST）、SLC 26 A3（CLD）和SLC 26 A4（PDS）可以不同特异性地转运氯离子、碘离子、碳酸氢根、草酸根和羟基阴离子，而SLC 26 A5（普雷斯廷）被认为是耳蜗外毛细胞的运动蛋白。我们报告了SLC 26家族的扩展，在染色体3，6，8，12和17中有5个新成员，并将SLC 26 A1定位到4p16.3。我们对其中之一SLC 26 A6进行了更详细的描述。它定位于染色体3p21.3，编码预测的738个氨基酸的跨膜蛋白，并且在肾脏和胰腺中表达最丰富。胰腺导管细胞系Capan-1和Capan-2表达SLC 26 A6，免疫组织化学将SLC 26 A6蛋白定位于胰腺导管细胞的顶端表面，表明其为管腔阴离子交换剂的候选者。功能。该组织特异性基因家族的新成员的表征可能为身体不同部位的阴离子转运生理学提供新的见解。(C)北京大学出版社.
A second distinct family of anion transporters, in addition to the classical SLC4 (or AE) family, has recently been delineated. Members of the SLC26 family are structurally well conserved and can mediate the electroneutral exchange of Cl- for HCO3- across the plasma membrane of mammalian cells like members of the SLC4 family. Three human transporter proteins have been functionally characterized: SLC26A2 (DTDST), SLC26A3 (CLD or DRA), and SLC26A4 (PDS) can transport with different specificities the chloride, iodine, bicarbonate, oxalate, and hydroxyl anions, whereas SLC26A5 (prestin) was suggested to act as the motor protein of the cochlear outer hair cell. We report the expansion of the SLC26 family with five new members in chromosomes 3, 6, 8, 12, and 17 and mapping of SLC26A1 to 4p16.3. We have characterized one of them, SLC26A6, in more detail. It maps to chromosome 3p21.3, encodes a predicted 738-amino-acid transmembrane protein, and is most abundantly ex pressed in the kidney and pancreas. Pancreatic ductal cell lines Capan-1 and Capan-2 express SLC26A6, and immunohistochemistry localizes SLC26A6 protein to the apical surface of pancreatic ductal cells, suggesting it as a candidate for a luminal anion exchanger. The functional. characterization of the novel members of this tissue-specific gene family may provide new insights into anion transport physiology in different parts of the body. (C) 2000 Academic Press.