The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog ftsZ2 To Control Cell Division in Archaea
The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog ftsZ2 To Control Cell Division in Archaea
复制标题
DOI：
10.1128/mbio.01007-20
复制
发表时间：
2020-07-01
期刊：
影响因子：
6.4
通讯作者：
Schmid, Amy K.
中科院分区：
文献类型：
--
作者：
Darnell, Cynthia L.;Zheng, Jenny;Schmid, Amy K.
细胞周期的精确控制是所有细胞生理学的核心。在先前的工作中，我们证明了古细菌细胞保持恒定的大小；然而，细胞周期的调控机制在这一生命领域仍未被探索。在这里，我们使用遗传学、功能基因组学和定量成像来鉴定和表征古细菌模型物种中新的CdrSL基因调控网络。我们证明了这些带状-螺旋-螺旋家族转录因子在细胞分裂调控中的核心作用，通过特定的转录控制基因编码FtsZ2，一个假定的微管蛋白同源物。利用延时荧光显微镜观察在微流体装置中培养的活细胞，我们进一步证明了FtsZ2是细胞分裂所必需的，而不是延伸所必需的。CdrS - ftsz2位点在整个古菌结构域高度保守，CdrS调控细胞分裂的核心功能在高盐古菌中是保守的。我们提出CdrSL-FtsZ2转录网络在古细菌中协调细胞分裂时间与细胞生长。健康的细胞生长和分裂对个体生物的生存和物种的长期生存能力至关重要。然而，尚不清楚域古菌的细胞如何维持健康的细胞周期。考虑到古细菌细胞是产生真核生物的内共生事件的宿主，了解古细菌细胞周期对进化具有至关重要的意义。在这里，我们鉴定和表征了调节古细菌细胞分裂所需的新分子参与者。这些分子决定细胞分裂的时间，但对于分裂之间的生长是必不可少的。时间是通过对细胞分裂环的转录控制来完成的。我们的结果揭示了古细菌细胞周期的机制，迄今为止仍然难以捉摸。
Precise control of the cell cycle is central to the physiology of all cells. In prior work we demonstrated that archaeal cells maintain a constant size; however, the regulatory mechanisms underlying the cell cycle remain unexplored in this domain of life. Here, we use genetics, functional genomics, and quantitative imaging to identify and characterize the novel CdrSL gene regulatory network in a model species of archaea. We demonstrate the central role of these ribbon-helix-helix family transcription factors in the regulation of cell division through specific transcriptional control of the gene encoding FtsZ2, a putative tubulin homolog. Using time-lapse fluorescence microscopy in live cells cultivated in microfluidics devices, we further demonstrate that FtsZ2 is required for cell division but not elongation. The cdrS-ftsZ2 locus is highly conserved throughout the archaeal domain, and the central function of CdrS in regulating cell division is conserved across hypersaline adapted archaea. We propose that the CdrSL-FtsZ2 transcriptional network coordinates cell division timing with cell growth in archaea.IMPORTANCE Healthy cell growth and division are critical for individual organism survival and species long-term viability. However, it remains unknown how cells of the domain Archaea maintain a healthy cell cycle. Understanding the archaeal cell cycle is of paramount evolutionary importance given that an archaeal cell was the host of the endosymbiotic event that gave rise to eukaryotes. Here, we identify and characterize novel molecular players needed for regulating cell division in archaea. These molecules dictate the timing of cell septation but are dispensable for growth between divisions. Timing is accomplished through transcriptional control of the cell division ring. Our results shed light on mechanisms underlying the archaeal cell cycle, which has thus far remained elusive.