The complete mitochondrial genome of Taxus cuspidata (Taxaceae): eight protein-coding genes have transferred to the nuclear genome
The complete mitochondrial genome of Taxus cuspidata (Taxaceae): eight protein-coding genes have transferred to the nuclear genome
复制标题
红豆杉科（Taxus cuspidata）完整线粒体基因组：8个蛋白质编码基因已转移至核基因组
DOI：
10.1186/s12862-020-1582-1
复制
发表时间：
2020-01-20
影响因子：
3.4
通讯作者：
Wang, Xiao-Quan
中科院分区：
文献类型：
--
作者：
Kan, Sheng-Long;Shen, Ting-Ting;Wang, Xiao-Quan
裸子植物代表了种子植物六个谱系中的五个。然而，大多数测序的植物线粒体基因组（有丝分裂基因组）已产生的被子植物，而有丝分裂基因组序列已产生的只有六个裸子植物。特别是，完整的有丝分裂基因组可用于所有主要的种子植物谱系，除了针叶树II（非松科针叶树或柏科植物），一个重要的谱系，包括六个家庭，这阻碍了全面了解有丝分裂基因组的多样性和裸子植物的进化。结果在这里，我们报告了完整的有丝分裂基因组的东北红豆杉针叶树II。与先前发表的裸子植物有丝分裂基因组比较，我们发现红豆杉和Welwitschia的有丝分裂基因组已经单独丢失了许多基因，而所有的基因在苏铁，银杏和松科的有丝分裂基因组中被鉴定。在裸子植物的某些谱系中，多个tRNA基因和内含子也丢失了，类似于在被子植物中观察到的模式。一般来说，裸子植物中的基因簇可能比被子植物中的保守性低。此外，在红豆杉和Welwitschia有丝分裂基因组中鉴定到的RNA编辑位点比在其他有丝分裂基因组中少，这可能与这两个物种中较少的内含子和频繁的基因丢失有关。结论我们完成了东北红豆杉细胞核分裂基因组的测序，并与其他四个裸子植物谱系的细胞核分裂基因组进行了比较。结果表明，裸子植物有丝分裂基因组在大小、结构、基因和内含子的含量、外源序列以及突变率等方面都具有不同于被子植物的多样性。
Background Gymnosperms represent five of the six lineages of seed plants. However, most sequenced plant mitochondrial genomes (mitogenomes) have been generated for angiosperms, whereas mitogenomic sequences have been generated for only six gymnosperms. In particular, complete mitogenomes are available for all major seed plant lineages except Conifer II (non-Pinaceae conifers or Cupressophyta), an important lineage including six families, which impedes a comprehensive understanding of the mitogenomic diversity and evolution in gymnosperms. Results Here, we report the complete mitogenome of Taxus cuspidata in Conifer II. In comparison with previously released gymnosperm mitogenomes, we found that the mitogenomes of Taxus and Welwitschia have lost many genes individually, whereas all genes were identified in the mitogenomes of Cycas, Ginkgo and Pinaceae. Multiple tRNA genes and introns also have been lost in some lineages of gymnosperms, similar to the pattern observed in angiosperms. In general, gene clusters could be less conserved in gymnosperms than in angiosperms. Moreover, fewer RNA editing sites were identified in the Taxus and Welwitschia mitogenomes than in other mitogenomes, which could be correlated with fewer introns and frequent gene losses in these two species. Conclusions We have sequenced the Taxus cuspidata mitogenome, and compared it with mitogenomes from the other four gymnosperm lineages. The results revealed the diversity in size, structure, gene and intron contents, foreign sequences, and mutation rates of gymnosperm mitogenomes, which are different from angiosperm mitogenomes.