Further evidence for the non-immunoglobulin nature of the bacterial aggregating factor in saliva.
Further evidence for the non-immunoglobulin nature of the bacterial aggregating factor in saliva.
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唾液中细菌聚集因子的非免疫球蛋白性质的进一步证据。
DOI：
10.1159/000260327
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发表时间：
1978
期刊：
影响因子：
4.2
通讯作者：
K. Guilmette
中科院分区：
文献类型：
--
作者：
S. Kashket;K. Guilmette
1972年)。当用SepharoseCL-2B(Pharmacia Fine Chemals，Inc.，Uppsala)上的层析对唾液进行分级时，发现聚集活性既存在于空隙体积区域(图2)，也存在于空隙体积区域(图2)。1，峰1)，并且在以大约一半的总柱体积洗脱的区域(峰2)。后一峰的活性约占从所有组分回收的总聚集活性的3%。然而，这一区域的比例在不同的实验之间是不同的，高达总比例的15%。样品经冷冻干燥、H20溶解后，用抗羊抗人IgA免疫扩散法(Miles-Yeda，Ltd.)检测。从空隙体积中分离出来的组分不能产生沉淀带。同样，直到第32号的所有组分都不含有任何可测量的IgA。然而，组分33-38提供了较强的沉淀线，在聚集试验中不起作用。沉淀线
1972]. When saliva was fractionated by chromatography on Sepharose CL-2B (Pharmacia Fine Chemicals, Inc., Uppsala), it was found that aggregating activity was present both in the void volume region (fig. 1, peak 1) and in a region that eluted at about one half of the total column volume (peak 2). The activity in the latter peak con stituted about 3% of the total aggregating activity recovered from all fractions. How ever, the fraction in this region was variable between experiments and was as high as about 15% of the total. The eluted samples were lyophilized, redissolved in H20 and assayed for IgA by immunodiffusion against goat anti-human IgA (Miles-Yeda, Ltd.). Fractions from the void volume failed to give precipitin bands. Similarly, none of the fractions up to No. 32 contained any meas urable IgA. Fractions 33-38, however, gave strong precipitin lines and were inactive in the aggregation assay. The precipitin lines