ELECTRON MICROSCOPICAL LOCALIZATION OF ZINC IN HIPPOCAMPAL MOSSY FIBRE SYNAPSES BY A MODIFIED SULFIDE SILVER PROCEDURE
ELECTRON MICROSCOPICAL LOCALIZATION OF ZINC IN HIPPOCAMPAL MOSSY FIBRE SYNAPSES BY A MODIFIED SULFIDE SILVER PROCEDURE
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DOI：
10.1007/bf00401978
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
HAUG, FMS
中科院分区：
文献类型：
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作者：
HAUG, FMS
Timm的硫化银法显示了重金属在脑和其他组织中的分布。该方法是非特异性的，但在海马区是已知的可视化集中在苔藓纤维系统中的锌。在这项研究中，该方法进行了修改，包括使用甲醛作为固定剂，以提供一个可容忍的程度的超微结构保存。电子致密的银颗粒被证明在苔藓纤维巨结内。它们均匀地分布在整个终扣上，但完全避开了线粒体。未观察到靠近突触裂隙或细胞膜其他部位的浓度。突触后元件（分叉棘或赘生物）缺乏银颗粒。
The sulfide silver method of Timm demonstrates the distribution of heavy metals in brain and other tissues. The method is unspecific, but in the hippocampal region is known to visualize the zinc concentrated in the mossy fibre system. In this study, the method was modified, including the use of formaldehyde as a fixative, to give a tolerable degree of ultrastructural preservation. Electrondense silver particles were demonstrated within mossy fibre giant boutons exclusively. They were evenly distributed throughout the boutons but avoided the mitochondria entirely. No concentration close to synaptic clefts or cell membranes elsewhere was observed. The postsynaptic elements (ramified spines or excrescences) were devoid of silver particles.