Glucose oxidase/hexokinase electrode for the determination of ATP
Glucose oxidase/hexokinase electrode for the determination of ATP
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DOI：
10.1016/s0003-2670(96)00451-5
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发表时间：
1997-03-20
影响因子：
6.2
通讯作者：
Guilbault, GG
中科院分区：
文献类型：
--
作者：
Compagnone, D;Guilbault, GG
将葡萄糖氧化酶和己糖激酶固定化，制成过氧化氢基ATP酶电极。酶之间对底物葡萄糖的竞争允许测量ATP。已经测试了不同的固定化程序和不同类型的己糖激酶。使用BSA-戊二醛程序和来自面包酵母的高产菌株的己糖激酶，ATP在0.05-0.5 mmol l（-1）范围内测量，检测限为0.01 mmol l（-1）。ATP浓度与文献报道的浓度相当，并获得了良好的回收率时，酶电极与人红细胞溶血液。
A hydrogen peroxide based enzyme electrode for the determination of ATP has been developed by the immobilization of glucose oxidase and hexokinase. Competition between the enzymes for the substrate glucose allowed the measurement of ATP. Different immobilization procedures and different types of hexokinase have been tested. Using a BSA-glutaraldehyde procedure and hexokinase from an overproducing strain of bakers' yeast, ATP was measured in the 0.05-0.5 mmol l(-1) range with a detection limit of 0.01 mmol l(-1). ATP concentrations comparable to those reported in the literature and a good recovery were obtained when the enzyme electrode was used with human erythrocyte hemolysate.