A Spatial Control for Correct Timing of Gene Expression during the Escherichia coli Cell Cycle.
A Spatial Control for Correct Timing of Gene Expression during the Escherichia coli Cell Cycle.
复制标题
大肠杆菌细胞周期中基因表达正确时间的空间控制
DOI：
10.3390/genes8010001
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发表时间：
2016-12-23
期刊：
影响因子：
3.5
通讯作者：
Morigen
中科院分区：
文献类型：
--
作者：
Yao Y;Fan L;Shi Y;Odsbu I;Morigen
基因的瞬时转录是通过不同的机制实现的，例如激活蛋白和阻遏蛋白与启动子的动态相互作用，以及作为细胞周期的函数的关键调控因子的积累和/或降解。我们发现，TorR蛋白定位于大肠杆菌细胞的旧极，形成一个功能集中。TorR焦点以细胞周期依赖性方式与类核共定位，并因此调节许多基因的转录。在细胞的旧极形成一个TorR焦点需要与MreB和DnaK蛋白以及ATP相互作用，这表明TorR递送需要细胞骨架组织和ATP。此外，缺乏蛋白质-蛋白质相互作用和ATP导致TorR作为转录因子的功能丧失。我们提出了一种机制，细胞周期依赖性基因转录的时间，其中转录因子与其靶基因相互作用，在细胞周期的特定时期，通过限制自己的空间分布。
Temporal transcriptions of genes are achieved by different mechanisms such as dynamic interaction of activator and repressor proteins with promoters, and accumulation and/or degradation of key regulators as a function of cell cycle. We find that the TorR protein localizes to the old poles of the Escherichia coli cells, forming a functional focus. The TorR focus co-localizes with the nucleoid in a cell-cycle-dependent manner, and consequently regulates transcription of a number of genes. Formation of one TorR focus at the old poles of cells requires interaction with the MreB and DnaK proteins, and ATP, suggesting that TorR delivery requires cytoskeleton organization and ATP. Further, absence of the protein–protein interactions and ATP leads to loss in function of TorR as a transcription factor. We propose a mechanism for timing of cell-cycle-dependent gene transcription, where a transcription factor interacts with its target genes during a specific period of the cell cycle by limiting its own spatial distribution.