Fluorescence Correlation Spectroscopy: Past, Present, Future
Fluorescence Correlation Spectroscopy: Past, Present, Future
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DOI：
10.1016/j.bpj.2011.11.012
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发表时间：
2011-12-21
影响因子：
3.4
通讯作者：
Elson, Elliot L.
中科院分区：
文献类型：
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作者：
Elson, Elliot L.
近年来，荧光相关光谱（FCS）已成为测定扩散系数、化学速率常数、分子浓度、荧光亮度、三重态寿命和其他分子参数的常规方法。FCS测量单个分子与其自身的空间和时间相关性，因此提供了经典系综和当代单分子测量之间的桥梁。它还提供非线性反应系统的浓度和分子数波动信息，补充单分子测量。FCS通常在荧光显微镜上实现，它可以对飞升体积进行采样，因此对于表征生物细胞等小型动态系统特别有用。然而，除了它的实际效用，FCS提供了一个窗口介观系统中，从稳定状态的波动不仅提供了测量的基础，但也可以有重要的后果的行为和系统的演变。例如，FCS研究的一个新的和潜在的有趣领域可能是非平衡稳态的研究，特别是在活细胞中。
In recent years fluorescence correlation spectroscopy (FCS) has become a routine method for determining diffusion coefficients, chemical rate constants, molecular concentrations, fluorescence brightness, triplet state lifetimes, and other molecular parameters. FCS measures the spatial and temporal correlation of individual molecules with themselves and so provides a bridge between classical ensemble and contemporary single-molecule measurements. It also provides information on concentration and molecular number fluctuations for nonlinear reaction systems that complement single-molecule measurements. Typically implemented on a fluorescence microscope, FCS samples femtoliter volumes and so is especially useful for characterizing small dynamic systems such as biological cells. In addition to its practical utility, however, FCS provides a window on mesoscopic systems in which fluctuations from steady states not only provide the basis for the measurement but also can have important consequences for the behavior and evolution of the system. For example, a new and potentially interesting field for FCS studies could be the study of nonequilibrium steady states, especially in living cells.