Immunohistochemical analysis of SOX6 expression in human brain tumors.
Immunohistochemical analysis of SOX6 expression in human brain tumors.
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DOI：
10.1007/bf02482186
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Toda, Masahiro
中科院分区：
文献类型：
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作者：
Ueda, Ryo;Yoshida, Kazunari;Toda, Masahiro
我们以前证明了发育调控基因SOX 6在神经胶质瘤细胞和胎儿脑中强烈表达，但在正常成人脑中仅微弱表达。最近的研究表明，脑肿瘤细胞可能与神经干/祖细胞共享抗原，信号系统和行为。为了检验这一命题的有效性，我们分析了SOX 6在各种人类中枢神经系统（CNS）肿瘤中的表达。免疫组化分析显示，星形胶质细胞和少突胶质细胞肿瘤表达SOX 6;神经元胶质细胞肿瘤（中枢神经细胞瘤）和胚胎性肿瘤（髓母细胞瘤），这是由多潜能干细胞前体细胞，也表现出高强度的SOX 6染色。相比之下，室管膜肿瘤（室管膜瘤和室管膜下瘤）、脑膜瘤和神经鞘瘤（均为分化良好的肿瘤）显示SOX 6无染色或仅微弱染色。这些结果表明，SOX 6可能在能够神经元和神经胶质分化的双能或多能细胞中表达，但在完全分化的细胞中不表达。SOX 6可能是一个有用的标志物，用于诊断肿瘤所产生的未成熟的双能细胞，可以分化成神经元和神经胶质细胞。
We previously demonstrated that the developmentally regulated gene, SOX6, is strongly expressed in glioma cells and in the fetal brain, but only faintly in the normal adult brain. Recent studies have indicated that brain tumor cells may share antigens, signaling systems, and behavior with neural stem/progenitor cells. To test the validity of this proposition, we analyzed the expression of SOX6 in various human central nervous system (CNS) tumors. Immunohistochemical analysis revealed that astrocytic and oligodendroglial tumors expressed SOX6; neuronal-glial cell tumors (central neurocytoma) and embryonal tumors (medulloblastoma), which arise from multipotential stem cell precursors, also showed a high intensity of SOX6 staining. In contrast, ependymal tumors (ependymoma and subependymoma), meningioma, and schwannoma, which are all well differentiated tumors, showed either no staining or only faint staining for SOX6. These results suggest that SOX6 may be expressed in bipotential or multipotential cells capable of neuronal and glial differentiation, but not in fully differentiated cells. SOX6 may be a useful marker for the diagnosis of tumors arising from immature bipotential cells that may differentiate into neuronal and glial cells.