Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination.
Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination.
复制标题
种子萌发过程中胚胎内质网形态的长期成像。
DOI：
10.1007/978-1-4939-7389-7_6
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Dzimitrowicz N
中科院分区：
文献类型：
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作者：
Dzimitrowicz N
随着时间的推移，在细胞水平上对植物胚胎进行成像在技术上具有挑战性，因为一旦去除了保护性种皮，胚胎必须在实验期间保持在显微镜载玻片上的活力和无压力。在这里，我们描述了一个程序和合适的仪器可视化，在几天内，内质网（ER）形态的变化与萌发inArabidopsis thalianaseeds的过程。此外，我们还提出了一个用户友好的图像分析工具，使微妙的扰动在ER网络进行测量。
Imaging plant embryos at the cellular level over time is technically challenging, since the embryo, once its protective seed coat is removed, must be kept viable and unstressed on a microscope slide for the duration of the experiment. Here we describe a procedure and suitable apparatus for the visualization, over several days, of changes in endoplasmic reticulum (ER) morphology associated with the process of germination inArabidopsis thalianaseeds. Moreover, we also present a user-friendly image analysis tool which enables subtle perturbations in the ER network to be measured.