DEMONSTRATION OF A PH GRADIENT ACROSS THE MUCUS LAYER ON THE SURFACE OF HUMAN GASTRIC-MUCOSA INVITRO
DEMONSTRATION OF A PH GRADIENT ACROSS THE MUCUS LAYER ON THE SURFACE OF HUMAN GASTRIC-MUCOSA INVITRO
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DOI：
10.1136/gut.23.6.513
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发表时间：
1982-01-01
期刊：
影响因子：
24.5
通讯作者：
TURNBERG, LA
中科院分区：
文献类型：
--
作者：
BAHARI, HMM;ROSS, IN;TURNBERG, LA
在先前的研究中，分别在体内和体外，在大鼠和兔胃底粘膜上的粘液中证明了H+浓度梯度，这些观察结果支持粘液-碳酸氢盐保护屏障的可能性。目前的研究表明，在体外人胃粘膜上的粘液具有类似的梯度。在粘液-上皮界面的最小平均H+浓度为1.1 × 10 - 6。10-4 mM（pH 6.96，n = 10）时，平均管腔浓度为5.6 mM（pH 2.25）。阿司匹林（10 mM）和N-乙酰半胱氨酸（306 mM）（5%）增加了最低粘液内H+浓度，梯度被40 mM（pH 1.4）的管腔H+浓度淹没。在人胃粘膜上的粘液中明显存在H+浓度梯度。潜在的破坏剂可能通过破坏这种粘液-碱性（假定为粘液-碳酸氢盐）屏障的一种或另一种组分而起作用。
In previous studies, a H+ concentration gradient was demonstrated across the mucus on rat and rabbit fundic mucosa, in vivo and in vitro, respectively, observations which support the possibility of a mucus-bicarbonate protective barrier. The present studies demonstrate a similar gradient across the mucus on human gastric mucosa in vitro. The minimum mean H+ concentration at the mucus-epithelium interface was 1.1 .times. 10-4 mM (pH 6.96, n = 10) when the mean luminal concentration was 5.6 mM (pH 2.25). Aspirin (10 mM) and N-acetyl cysteine (306 mM) (5%) increased the minimum intramucus H+ concentration and the gradient was overwhelmed by a luminal H+ concentration of 40 mM (pH 1.4). A H+ concentration gradient apparently exists across the mucus on human gastric mucosa. Potential damaging agents may act by compromising one or another of the components of this mucus-alkaline, presumed mucus-bicarbonate, barrier.